Department of Biochemistry, Islamic Azad University, Tehran North Branch, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2023 Mar 1;24(3):833-840. doi: 10.31557/APJCP.2023.24.3.833.
Breast cancer is one of the important factors of cancer-related deaths. Considering the drug resistance, special attention has been paid to natural compounds. This study aimed at evaluating the anti-metastatic activity of fennel in a breast cancer mouse model.
A total of 35 adult female BALB/C mice were used in this study. Breast cancer was induced by subcutaneous injection of 4T1 cells in the right lower flank. The mice received fennel extracts daily via intraperitoneal injection for two weeks. Meanwhile, tumor volume was measured every day using calipers. After two weeks, each animal was anesthetized. The protein expression of HSP 70 & 90 was measured in liver tissue and ovary. The expression of her2 was measured in tumor tissue. The activity of Glutathione peroxidase and reductase as anti-oxidant agents were measured in serum.
Tumor size significantly decreased after nine days' treatment of the fennel. The expression of HER2 increased in the tumor tissue and decrease with different dose of fennel. Fennel treatment caused a decrease in the protein expression of HSP 70 & 90 in the liver tissues.
Based on our findings, fennel has anti-tumor and anti-metastatic activities against aggressive cancers.
乳腺癌是癌症相关死亡的重要因素之一。考虑到药物耐药性，人们特别关注天然化合物。本研究旨在评估茴香在乳腺癌小鼠模型中的抗转移活性。
本研究共使用了 35 只成年雌性 BALB/C 小鼠。通过在右下腹皮下注射 4T1 细胞诱导乳腺癌。小鼠通过腹腔注射每天接受茴香提取物治疗两周。同时，每天使用卡尺测量肿瘤体积。两周后，对每只动物进行麻醉。测量肝组织和卵巢中 HSP 70 和 90 的蛋白表达。测量肿瘤组织中 her2 的表达。测量血清中作为抗氧化剂的谷胱甘肽过氧化物酶和还原酶的活性。
茴香治疗九天后肿瘤大小显著减小。肿瘤组织中 HER2 的表达增加，而随着茴香剂量的不同而减少。茴香处理导致肝组织中 HSP 70 和 90 的蛋白表达减少。
根据我们的发现，茴香具有抗肿瘤和抗侵袭性癌症转移的活性。
