Department of Otorhinolaryngology, Head and Neck Surgery, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.
Dessau Medical Center and Brandenburg Medical School Theodor Fontane, Dessau-Roßlau, Germany.
Anticancer Res. 2023 Apr;43(4):1437-1448. doi: 10.21873/anticanres.16292.
BACKGROUND/AIM: Urothelial carcinoma (UC) of the urinary bladder is the second most common tumor in the field of urology and is characterized by a relatively aggressive growth behavior. New therapeutic approaches are required to improve the prognosis of affected patients. We hypothesized a link between dysregulation of eIFs and the development of UC. Therefore, in the present work, we investigated the expression behavior of eIF1, eIF1AY, eIF1AX, eIF2α, eIF3a, eIF3b, eIF4B, eIF4E, eIF4G, eIF5A, eIF5B, and eIF6 in UC compared with that in urothelial tissue.
Paraffin-embedded tumor tissue samples from 107 patients suffering from UC were examined. Seventy-six patients contained adjacent urothelial tissue. Three tumor tissue cylinders (tumor collective) and two urothelial tissue cylinders (control collective) were collected per patient and embedded in tissue microarray (TMA) blocks. Immunohistochemical staining of the TMA sections was then performed. The staining results were assessed semi-quantitatively. Staining intensities and immunoreactive scores (IRS) of both collectives were compared. In each case, a distinction was made between cytoplasmic and nuclear staining.
Significant up-regulation of eIF1AY, eIF2α, eIF3a, eIF3b, eIF4B, eIF4G, eIF5B, and eIF6 was found in the cytoplasm of UC. In contrast, eIF1 and eIF5A were significantly down-regulated in the cytoplasm of UC. eIF5A and eIF6 were significantly down-regulated in the nuclei of UC.
Dysregulation of eIFs in the urothelium of the urinary bladder is linked to carcinogenesis at this site.
背景/目的：膀胱癌是泌尿外科领域第二大常见肿瘤，其生长行为具有相对侵袭性。需要新的治疗方法来改善受影响患者的预后。我们假设 eIFs 的失调与 UC 的发展之间存在联系。因此，在本研究中，我们研究了 eIF1、eIF1AY、eIF1AX、eIF2α、eIF3a、eIF3b、eIF4B、eIF4E、eIF4G、eIF5A、eIF5B 和 eIF6 在 UC 中的表达行为与尿路上皮组织中的表达行为进行了比较。
对 107 例 UC 患者的石蜡包埋肿瘤组织样本进行了检查。76 例患者包含相邻的尿路上皮组织。每位患者采集 3 个肿瘤组织圆柱体（肿瘤集合）和 2 个尿路上皮组织圆柱体（对照集合），并将其嵌入组织微阵列（TMA）块中。然后对 TMA 切片进行免疫组织化学染色。对染色结果进行半定量评估。比较两个集合的染色强度和免疫反应性评分（IRS）。在每种情况下，均对细胞质和核染色进行区分。
在 UC 的细胞质中发现 eIF1AY、eIF2α、eIF3a、eIF3b、eIF4B、eIF4G、eIF5B 和 eIF6 的表达显著上调。相比之下，UC 细胞质中的 eIF1 和 eIF5A 表达显著下调。UC 细胞核中的 eIF5A 和 eIF6 表达显著下调。
膀胱尿路上皮中 eIFs 的失调与该部位的癌变有关。
