Caprodossi Sara, Lucciarini Roberta, Amantini Consuelo, Nabissi Massimo, Canesin Giacomo, Ballarini Patrizia, Di Spilimbergo Adriana, Cardarelli Marco Andrea, Servi Lucilla, Mammana Gabriele, Santoni Giorgio
Department of Experimental Medicine and Public Health, University of Camerino, Camerino (MC), Italy.
Eur Urol. 2008 Sep;54(3):612-20. doi: 10.1016/j.eururo.2007.10.016. Epub 2007 Oct 16.
To evaluate the expression of transient receptor potential vanilloid type 2 (TRPV2) in normal human bladder and urothelial carcinoma (UC) tissues.
Bladder specimens were obtained by transurethral resection or radical cystectomy. TRPV2 mRNA expression in normal human urothelial cells (NHUCs), UC cell lines, and formalin-fixed paraffin-embedded normal (n=6) and cancer bladder tissues (n=58) was evaluated by polymerase chain reaction (PCR) and quantitative real-time PCR (RT-PCR). TRPV2 protein expression was assessed by cytofluorimetric and confocal microscopy analyses in NHUCs and UC cells and by Western blotting and immunohistochemistry in normal and UC tissues.
Enhanced TRPV2 mRNA and protein expression was found in high-grade and -stage UC specimens and UC cell lines. Both the full-length TRPV2 (hTRPV2) and a short splice-variant (s-TRPV2) were detected in NHUC and normal bladder specimens, whereas a progressive decline of s-TRPV2 in pTa, pT1, and pT2 stages was observed, up to a complete loss in pT3 and pT4 UC specimens.
Normal human urothelial cells and bladder tissue specimens express TRPV2 at both the mRNA and protein levels. A progressive loss of s-TRPV2 accompanied by a marked increase of hTRPV2 expression was found in high-grade and -stage UC tissues.
评估瞬时受体电位香草酸亚型2（TRPV2）在正常人类膀胱组织和尿路上皮癌（UC）组织中的表达情况。
通过经尿道切除术或根治性膀胱切除术获取膀胱标本。采用聚合酶链反应（PCR）和定量实时PCR（RT-PCR）评估正常人类尿路上皮细胞（NHUCs）、UC细胞系以及福尔马林固定石蜡包埋的正常膀胱组织（n = 6）和癌组织（n = 58）中TRPV2 mRNA的表达。通过细胞荧光分析和共聚焦显微镜分析评估NHUCs和UC细胞中TRPV2蛋白的表达，并通过蛋白质免疫印迹法和免疫组织化学法评估正常组织和UC组织中TRPV2蛋白的表达。
在高级别和晚期UC标本及UC细胞系中发现TRPV2 mRNA和蛋白表达增强。在NHUC和正常膀胱标本中均检测到全长TRPV2（hTRPV2）和一种短剪接变体（s-TRPV2），而在pTa、pT1和pT2期观察到s-TRPV2逐渐下降，在pT3和pT4 UC标本中完全缺失。
正常人类尿路上皮细胞和膀胱组织标本在mRNA和蛋白水平均表达TRPV2。在高级别和晚期UC组织中发现s-TRPV2逐渐缺失，同时hTRPV2表达显著增加。
