Paul Provakar, Samanta Saikat, Chatterjee Arunavo, Mallick Arabinda, Majumdar Tapas
Department of Chemistry, University of Kalyani, Nadia, West Bengal, India, 741235.
Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) - Kolkata, Mohanpur, West Bengal, India, 741246.
Phys Chem Chem Phys. 2023 Apr 5;25(14):10166-10174. doi: 10.1039/d3cp00105a.
Disaggregation-induced emission enhancement was studied using a self-aggregated bis-indole derivative, 3,3'-bisindolyl(phenyl)methane (BIPM), and β-CD molecules were employed for the emission recovery. In our recent study, BIPM molecules were found to exhibit weak emission efficiency in pure water due to aggregation-caused quenching (ACQ) effects. In the present study, we employed a simple, effective, biologically benign, and sustainable strategy in an attempt to disaggregate the BIPM self-aggregates into monomers to restore their emission efficiency. The β-CD molecules were found to be effective in disaggregating the BIPM associations through tugging the monomers from their self-associations and encapsulating them into supramolecular nanocavities. The changes in the photophysical, dynamical, and thermodynamic properties associated with the disaggregation of the probe assemblies were studied by employing steady-state and time-resolved spectroscopy, isothermal titration calorimetry, and transmission electron microscopy with support from computational studies. The detailed photophysical and thermodynamic investigations on the disaggregation of the BIPM self-associations might provide significant insights towards its suitability for diverse biological and pharmaceutical applications.
使用一种自聚集的双吲哚衍生物3,3'-双吲哚基（苯基）甲烷（BIPM）研究了聚集诱导发光增强现象，并采用β-环糊精（β-CD）分子来恢复其发光。在我们最近的研究中，发现BIPM分子在纯水中由于聚集导致猝灭（ACQ）效应而表现出较弱的发光效率。在本研究中，我们采用了一种简单、有效、生物相容性好且可持续的策略，试图将BIPM自聚集体解聚为单体以恢复其发光效率。发现β-CD分子通过将单体从其自缔合中拉出并将它们封装到超分子纳米腔中来有效地解聚BIPM缔合体。通过稳态和时间分辨光谱、等温滴定量热法以及透射电子显微镜，并在计算研究的支持下，研究了与探针组装体解聚相关的光物理、动力学和热力学性质的变化。对BIPM自缔合解聚的详细光物理和热力学研究可能为其在各种生物和制药应用中的适用性提供重要见解。
