Rojek Sebastian, Poljańska Ewa, Chaim Weronika, Maciów-Głąb Martyna, Bystrowska Beata
Department of Forensic Medicine, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16 Str., 31-531 Kraków, Poland.
Toxicology Research Group, Department of Toxicology, Jagiellonian University Medical College, Medyczna 9 Str., 30-688 Krakow, Poland.
Toxics. 2023 Feb 25;11(3):220. doi: 10.3390/toxics11030220.
Legal highs present a great threat to health, especially in groups of people experimenting with psychoactive substances. The lack of available knowledge on the biotransformation of these substances necessitates symptomatic treatment in the event of intoxication, which, unfortunately, may be ineffective. Opioids, including heroin analogues, such as U-47700, constitute a special group of designer drugs. In this study, a multi-directional approach to trace the biotransformation of U-47700 in living organisms was used. For this purpose, the in silico assessment (ADMET Predictor) was used first and then followed by an in vitro study using human liver microsomes and the S9 fraction. The biotransformation was then followed in an animal model (Wistar rats). Tissues such as blood, brain and liver were collected for analysis. The study was performed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The obtained results were compared to those obtained from the analysis of autopsy materials (cases analysed in the Toxicology Laboratory of the Department of Forensic Medicine, Jagiellonian University Medical College in Krakow).
新型毒品对健康构成巨大威胁，尤其是在尝试使用精神活性物质的人群中。由于缺乏关于这些物质生物转化的现有知识，中毒时需要进行对症治疗，但不幸的是，这种治疗可能无效。阿片类药物，包括海洛因类似物，如U-47700，构成了一类特殊的合成毒品。在本研究中，采用了多方向方法来追踪U-47700在生物体内的生物转化。为此，首先使用计算机模拟评估（ADMET Predictor），然后进行使用人肝微粒体和S9组分的体外研究。随后在动物模型（Wistar大鼠）中追踪生物转化过程。收集血液、大脑和肝脏等组织进行分析。该研究使用液相色谱-串联质谱法（LC-MS/MS）进行。将获得的结果与从尸检材料分析中获得的结果进行比较（这些病例在克拉科夫雅盖隆大学医学院法医学系毒理学实验室进行分析）。
