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意大利南部鸬鹚(Blumenbach, 1978)体内异尖线虫属(线虫纲:异尖科)的鉴定

Identification of (Nematoda: Anisakidae) in Great Cormorants (Blumenbach, 1978) from Southern Italy.

作者信息

Cammilleri Gaetano, D'Amelio Stefano, Ferrantelli Vincenzo, Costa Antonella, Buscemi Maria Drussilla, Castello Annamaria, Bacchi Emanuela, Goffredo Elisa, Mancini Maria Emanuela, Cavallero Serena

机构信息

Centro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Via Gino Marinuzzi 3, 90129 Palermo, Italy.

Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

出版信息

Vet Sci. 2023 Mar 4;10(3):194. doi: 10.3390/vetsci10030194.

Abstract

In this study, four dead great cormorant (Blumenbach, 1978) specimens, collected from the coasts and lakes of Southern Italy, were examined by necropsy for the detection of sp. The adults and larvae found were subjected to morphological analysis and molecular identification by PCR-RFLP. A total of 181 specimens were detected in all of the four great cormorants examined (prevalence = 100%), showing an intensity of infestation between nine and ninety-two. A co-infestation by adult and larval forms of was found only in one of the great cormorants examined. Following molecular investigations, 48 specimens of A and 38 specimens of B were detected, revealing co-infestation solely for the great cormorant from Leporano Bay (Southern Italy). Our results showed an opposite ratio between A and B in Pantelleria and in Salso Lake (Southern Italy) compared to what was reported in the literature, probably due to migratory stopovers and the ecology of the infested fish species, confirming the role of nematodes as ecological tags of their hosts.

摘要

在本研究中,对从意大利南部海岸和湖泊采集的四只死亡的普通鸬鹚(布鲁门巴哈,1798年)标本进行了尸检,以检测吸虫。对发现的成虫和幼虫进行了形态分析,并通过PCR-RFLP进行分子鉴定。在所有四只接受检查的普通鸬鹚中总共检测到181个标本(感染率 = 100%),感染强度在9至92之间。仅在一只接受检查的普通鸬鹚中发现了成虫和幼虫形式的混合感染。经过分子研究,检测到48个A种标本和38个B种标本,结果表明只有来自莱波拉诺湾(意大利南部)的普通鸬鹚存在混合感染。我们的结果显示,与文献报道相比,潘泰莱里亚岛和萨尔索湖(意大利南部)的A种和B种吸虫比例相反,这可能是由于迁徙中途停留以及受感染鱼类物种的生态习性所致,证实了线虫作为其宿主生态标记的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e3f/10057129/e59354784e73/vetsci-10-00194-g001.jpg

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