School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, PR China.
School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, PR China.
Int J Biol Macromol. 2023 May 15;237:124212. doi: 10.1016/j.ijbiomac.2023.124212. Epub 2023 Mar 26.
To augment the functional attributes of pectin and expand its prospective utilization in food preservation, this research explored the enzymatic grafting of resorcinol and 4-hexylresorcinol onto pectin. Structural analysis verified the successful grafting of both resorcinol and 4-hexylresorcinol to pectin via esterification, with the 1-OH of resorcinol and 4-hexylresorcinol and the carboxyl group of pectin functioning as grafting sites. The grafting ratios of resorcinol-modified pectin (Re-Pe) and 4-hexylresorcinol-modified pectin (He-Pe) were 17.84 % and 10.98 %, respectively. This grafting modification notably enhanced the antioxidative and antibacterial properties of pectin. Specifically, DPPH clearance and the inhibition ratio in the β-carotene bleaching assay increased from 11.38 % and 20.13 % (native pectin, Na-Pe) to 41.15 % and 36.67 % (Re-Pe), and 74.72 % and 53.40 % (He-Pe). Moreover, the inhibition zone diameter against Escherichia coli and Staphylococcus aureus rose from 10.12 and 10.08 mm (Na-Pe) to 12.36 and 11.52 mm (Re-Pe), and 16.78 and 14.87 mm (He-Pe). Additionally, the application of native and modified pectin coatings effectively impeded pork spoilage, with the modified pectins demonstrating a more potent effect. Among the two modified pectins, He-Pe exhibited the most significant enhancement in pork shelf life.
为了增强果胶的功能属性，并拓展其在食品保鲜中的应用前景，本研究探索了通过酶促反应将间苯二酚和 4-己基间苯二酚接枝到果胶上。结构分析证实了间苯二酚和 4-己基间苯二酚成功地通过酯化反应接枝到果胶上，间苯二酚的 1-OH 和 4-己基间苯二酚以及果胶的羧基作为接枝位点。接枝到果胶上的间苯二酚的接枝率（Re-Pe）和 4-己基间苯二酚的接枝率（He-Pe）分别为 17.84%和 10.98%。这种接枝修饰显著增强了果胶的抗氧化和抗菌性能。具体而言，DPPH 清除率和β-胡萝卜素漂白抑制率从 11.38%和 20.13%（天然果胶，Na-Pe）分别增加到 41.15%和 36.67%（Re-Pe），和 74.72%和 53.40%（He-Pe）。此外，对大肠杆菌和金黄色葡萄球菌的抑制带直径从 10.12 和 10.08mm（Na-Pe）分别增加到 12.36 和 11.52mm（Re-Pe），和 16.78 和 14.87mm（He-Pe）。此外，天然和改性果胶涂层的应用有效地阻止了猪肉变质，改性果胶表现出更强的效果。在两种改性果胶中，He-Pe 对猪肉货架期的延长效果最为显著。
