Fellowship in Endodontics Program, Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Clin Oral Investig. 2023 May;27(5):2235-2243. doi: 10.1007/s00784-023-04960-0. Epub 2023 Mar 28.
To determine the impact of heat exposure of different sealers on inflammatory cytokine secretions and tissue response in vivo.
Silicone tubes were prefilled with epoxy resin (ER) or calcium silicate (CS) sealers, preheated at 37, 60, or 120 °C, and implanted in rat subcutaneous site. Peri-implant exudate and tissue were analyzed after 1 and 4 weeks for cytokine secretions and tissue organization.
At 1 week, 120 °C-preheated CS and ER induced higher secretions of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6), respectively, as compared to sham/empty tube groups. At 4 weeks, whereas TNF-α secretion was reduced in CS, it increased in ER group, particularly for 120 °C. Both sealers revealed high IL-6 after 4 weeks as compared to sham/empty tube, and generally, higher IL-6 secretions were associated with ER. Histology at 1 week revealed lower degree of inflammatory infiltrate in the groups of the highest preheating temperature (120 °C). Nonetheless, at 4 weeks, whereas fibrous capsule area and inflammatory infiltrate remained low in the CS120 group, they were high in ER120.
Preheating ER sealer to 120 °C induced high and prolonged secretion of proinflammatory cytokines (TNF-α and IL-6), whereas this effect was transient for the CS sealer. This was associated with increased fibrous capsule and inflammatory infiltrate in response to 120 °C-preheated ER.
Heat-induced changes in sealer properties alter the inflammatory response in vivo, which may affect the clinical outcome. This will not only help appropriate selection of obturation technique for different sealers, but also for optimizing the properties of new generation of sealers.
确定不同密封剂暴露于热时对体内炎症细胞因子分泌和组织反应的影响。
将环氧树脂（ER）或硅酸钙（CS）密封剂预填充到硅胶管中，在 37、60 或 120°C 下预热，并植入大鼠皮下部位。在 1 周和 4 周时，分析植入部位周围渗出物和组织中细胞因子的分泌和组织学变化。
在 1 周时，与 sham/empty 管组相比，120°C 预热的 CS 和 ER 分别诱导了更高水平的肿瘤坏死因子-α（TNF-α）和白细胞介素 6（IL-6）的分泌。在 4 周时，CS 组的 TNF-α分泌减少，而 ER 组则增加，特别是在 120°C 时增加。与 sham/empty 管相比，两种密封剂在 4 周时均显示出较高的 IL-6 分泌，且一般而言，较高的 IL-6 分泌与 ER 相关。在 1 周时的组织学观察显示，在预热温度最高（120°C）的组中，炎症浸润程度较低。然而，在 4 周时，CS120 组的纤维囊区和炎症浸润仍然较低，而 ER120 组则较高。
将 ER 密封剂预热至 120°C 可诱导高水平和长时间的促炎细胞因子（TNF-α和 IL-6）分泌，而 CS 密封剂的这种作用是短暂的。这与 120°C 预热 ER 后纤维囊和炎症浸润的增加有关。
密封剂性质的热诱导变化改变了体内的炎症反应，这可能会影响临床结果。这不仅有助于为不同的密封剂选择合适的封闭技术，还有助于优化新一代密封剂的性能。
