Hagar Manal A, El Sharkawy Asmaa Z, Abd-Allah Ghada E, Kadada Hala M, Farghaly D S
Pak J Biol Sci. 2022 Nov;25(12):1058-1065. doi: 10.3923/pjbs.2022.1058.1065.
<b>Background and Objective:</b> The cotton leafworm, <i>Spodoptera littoralis</i> (Boisd.) (Lepidoptera: Noctuidae), represents the worst lepidopterous pest on most vegetable crops. Moreover, its host range is extensive because it is a polyphagous and cosmopolitan pest that can cause an estimated loss of nearly 25.8 to 100% of crop production. Natural controls have replaced pesticides due to the environmental damage that chemical pesticides cause. Eggplant, tomato, okra and pepper are Egypt's most significant vegetable crops and the leaves of these plants are cheap and can act as botanical and nontoxic pesticides. <b>Materials and Methods:</b> <i>Spodoptera littoralis </i>larvae were reared in the laboratory and plant leaves of eggplant, tomato, okra and pepper were extracted in the laboratory with organic solvents. Then, these extracts were applied to the larvae. Therefore, the histological sectors in larvae bodies were prepared and the changes were observed compared with the control. <b>Results:</b> This study proved the efficiency of all plant leaf extracts, especially those of eggplant and tomato leaves (LC<sub>50</sub> of 1191.9 and 1582.7 ppm, respectively). In contrast, okra and pepper leaf extracts had the highest LC<sub>50</sub> (3904.8 and 5431.6 ppm, respectively). Also, the abnormalities in the midgut and internal tissues were evident in the larvae treated with eggplant leaf extracts, followed by tomato leaf extracts and okra leaf extracts. Larvae treated with pepper leaf extracts were as normal as the control. <b>Conclusion:</b> The current study opens horizons to new plant extracts that are cheap and unused plant parts and make them helpful as new and good insecticides.
背景与目的:棉铃虫,即埃及棉铃虫(Spodoptera littoralis)(博伊斯德)(鳞翅目:夜蛾科),是大多数蔬菜作物上最严重的鳞翅目害虫。此外,它的寄主范围广泛,因为它是一种多食性且世界性分布的害虫,估计会导致农作物产量损失近25.8%至100%。由于化学农药对环境造成破坏,自然防治方法已取代了农药。茄子、番茄、秋葵和辣椒是埃及最重要的蔬菜作物,这些植物的叶子价格低廉,可作为植物性无毒农药。材料与方法:在实验室饲养埃及棉铃虫幼虫,并在实验室用有机溶剂提取茄子、番茄、秋葵和辣椒的植物叶子。然后,将这些提取物应用于幼虫。因此,制备了幼虫体内的组织切片,并与对照组比较观察变化情况。结果:本研究证明了所有植物叶提取物的有效性,尤其是茄子叶和番茄叶提取物(LC50分别为1191.9和1582.7 ppm)。相比之下,秋葵叶和辣椒叶提取物的LC50最高(分别为3904.8和5431.6 ppm)。此外,用茄子叶提取物处理的幼虫中肠和内部组织出现明显异常,其次是番茄叶提取物和秋葵叶提取物处理的幼虫。用辣椒叶提取物处理的幼虫与对照组一样正常。结论:当前研究为廉价且未被利用的植物新提取物开辟了前景,使其有望成为新型优质杀虫剂。