Huang Bocheng, Shi Menglin, Pang Aobo, Tan Beiping, Xie Shiwei
Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.
Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, China.
Animals (Basel). 2023 Mar 18;13(6):1090. doi: 10.3390/ani13061090.
In this study, we present data from an eight-week growth trial with pearl gentian grouper fed either a reference diet (FM) with a fishmeal level of 50%, or test diet wherein 15% (CAP15), 30% (CAP30), 45% (CAP45), and 60% (CAP60) fishmeal was replaced by protein meal (CAP). Results showed that the weight gain and daily feed intake ratio of CAP60 were significantly lower than the FM group. In the serum, compared to the FM group, the content of malondialdehyde (MDA), the activities of alanine aminotransferase in CAP60 and CAP45 groups, and acid phosphatase in the CAP60 group were significantly higher, while the content of total cholesterol in CAP60 and CAP45 groups was significantly lower. In the liver, compared to the control group, the content of MDA in the CAP60 group was significantly higher. in CAP30 to CAP60 groups and in CAP60 were significantly upregulated. In distal intestines, the activities of trypsin and superoxide dismutase of CAP30 to CAP60 groups were significantly lower than the FM group. In conclusion, for pearl gentian grouper, CAP could replace up to 45% of the fishmeal in the feed, while a 60% replacement level will affect cholesterol bile acid metabolism and health.
在本研究中，我们展示了珍珠龙胆石斑鱼为期八周的生长试验数据，试验中石斑鱼分别投喂鱼粉含量为50%的对照饲料（FM），或用蛋白饲料（CAP）替代15%（CAP15）、30%（CAP30）、45%（CAP45）和60%（CAP60）鱼粉的试验饲料。结果表明，CAP60组的体重增加和日采食量比例显著低于FM组。血清中，与FM组相比，CAP60和CAP45组的丙二醛（MDA）含量、CAP60组的谷丙转氨酶活性以及CAP60组的酸性磷酸酶活性显著更高，而CAP60和CAP45组的总胆固醇含量显著更低。肝脏中，与对照组相比，CAP60组的MDA含量显著更高。CAP30至CAP60组以及CAP60组中相关指标显著上调。在远端肠道中，CAP30至CAP60组的胰蛋白酶和超氧化物歧化酶活性显著低于FM组。总之，对于珍珠龙胆石斑鱼，CAP可替代饲料中高达45%的鱼粉，而60%的替代水平会影响胆固醇胆汁酸代谢及健康。
