HER2 模棱两可（2+）免疫组化病例中 mRNA 表达的评估。

Evaluation of mRNA Expression in HER2-Equivocal (2+) Immunohistochemistry Cases.

作者信息

Carretero-Barrio Irene, Caniego-Casas Tamara, Rosas Marta, Sánchez María Concepción, Martínez-Jáñez Noelia, Chiva Miguel, Sarrió David, Moreno-Bueno Gema, Palacios José, Pérez-Mies Belén

机构信息

Servicio de Anatomía Patológica, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain.

Faculty of Medicine, Universidad de Alcalá, 28801 Alcalá de Henares, Spain.

出版信息

Cancers (Basel). 2023 Mar 9;15(6):1688. doi: 10.3390/cancers15061688.

Xpert Breast Cancer STRAT4 is a RT-qPCR platform that studies the mRNA expression of , , and , providing a positive or negative result for each of these breast cancer biomarkers. Its concordance with immunohistochemistry (IHC) and in situ hybridization (ISH) has been previously demonstrated, but none of the previous works was focused on HER2-equivocal (2+) cases identified by IHC. Thus, we studied the concordance between IHC/ISH and STRAT4 results for 112 HER2 2+ IBC samples, using 148 HER2 0+, 1+ and 3+ (no-HER2 2+) samples for comparison. We found 91.3% accuracy for the determination of HER2 status globally, 99.3% for no-HER2 2+ samples and 80.7% for HER2 2+ samples. Regarding the other biomarkers, we obtained 96.4% accuracy for estrogen receptor, 84.1% for progesterone receptor and 58.2% for Ki67. Our results suggest that the use of mRNA for the evaluation of HER2 2+ cases is not a reliable reflex method to assess the amplification status.

Xpert乳腺癌STRAT4是一种逆转录定量聚合酶链反应（RT-qPCR）平台，可研究[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]的mRNA表达情况，为每种乳腺癌生物标志物提供阳性或阴性结果。此前已证明其与免疫组织化学（IHC）和原位杂交（ISH）的一致性，但之前的研究均未聚焦于免疫组织化学鉴定的HER2模棱两可（2+）病例。因此，我们研究了112例HER2 2+浸润性乳腺癌（IBC）样本的免疫组织化学/原位杂交与STRAT4结果之间的一致性，并使用148例HER2 0+、1+和3+（非HER2 2+）样本进行比较。我们发现，总体上HER2状态测定的准确率为91.3%，非HER2 2+样本为99.3%，HER2 2+样本为80.7%。对于其他生物标志物，雌激素受体的准确率为96.4%，孕激素受体为84.1%，Ki67为58.2%。我们的结果表明，使用[此处原文缺失具体基因名称]mRNA评估HER2 2+病例并非评估[此处原文缺失具体基因名称]扩增状态的可靠反射方法。