Department of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, Italy.
Biomeets Consulting, Carrer d'Àlaba, 61, 08005 Barcelona, Spain.
Genes (Basel). 2023 Feb 21;14(3):535. doi: 10.3390/genes14030535.
Climate change represents the main problem for agricultural crops, and the constitution of heat-tolerant genotypes is an important breeder's strategy to reduce yield losses. The aim of the present study was to investigate the whole genome of a heat-tolerant tomato genotype (E42), in order to identify candidate genes involved in its response to high temperature. E42 presented a high variability for chromosomes 1, 4, 7 and 12, and phylogenetic analysis highlighted its relationship with the wild species. Variants with high (18) and moderate (139) impact on protein function were retrieved from two lists of genes related to heat tolerance and reproduction. This analysis permitted us to prioritize a subset of 35 candidate gene mapping in polymorphic regions, some colocalizing in QTLs controlling flowering in tomato. Among these genes, we identified 23 HSPs, one HSF, six involved in flowering and five in pollen activity. Interestingly, one gene coded for a flowering locus T1 and mapping on chromosome 11 resides in a QTL region controlling flowering and also showed 100% identity with an allele. This study provides useful information on both the E42 genetic background and heat stress response, and further studies will be conducted to validate these genes.
气候变化是农业作物面临的主要问题，培育耐热基因型是减少产量损失的重要育种策略。本研究旨在研究耐热番茄基因型（E42）的全基因组，以鉴定参与其高温响应的候选基因。E42 染色体 1、4、7 和 12 高度变异，系统发育分析突出了其与野生种的关系。从与耐热和繁殖相关的两个基因列表中检索到具有高（18）和中（139）蛋白功能影响的变体。该分析使我们能够对一组 35 个候选基因进行优先级排序，这些基因映射在控制番茄开花的 QTL 中的多态性区域。在这些基因中，我们鉴定出 23 个 HSP、1 个 HSF、6 个参与开花和 5 个参与花粉活性的基因。有趣的是，一个编码开花位点 T1 的基因位于控制开花的染色体 11 的 QTL 区域，并且与一个 等位基因具有 100%的同一性。这项研究提供了有关 E42 遗传背景和耐热性响应的有用信息，并且将进一步进行研究以验证这些基因。
