Department of Forensic Medicine, University of Oslo, 0315 Oslo, Norway.
National Center for Forensic Science, University of Central Florida, Orlando, FL 32826, USA.
Genes (Basel). 2023 Mar 3;14(3):636. doi: 10.3390/genes14030636.
The ability to associate a contributor with a specific body fluid in a crime stain can aid casework investigation. The detection of body fluids combined with DNA analyses may supply essential information, but as the two tests are independent, they may not be associated. Recently, the analysis of coding region SNPs (cSNPs) within the RNA transcript has been proven to be a promising method to face this challenge. In this study, we performed targeted RNA sequencing of 158 samples (boxershorts, fingernail swabs and penile swabs) collected from 12 couples at different time points post-intimate contact and after non-intimate contact, using the Ion S5™ System and BFID-cSNP-6F assay. The aim of the study was to compare the performance of the MPS and CE methods in the detection of mRNA markers, and to associate body fluids with contributors by their cSNP genotypes. The results of the study show a lower success rate in the detection of vaginal mucosa by the MPS compared to the CE method. However, the additional information obtained with the cSNP genotypes could successfully associate body fluids with contributors in most cases.
将贡献者与犯罪痕迹中的特定体液相关联的能力可以辅助案件调查。体液检测与 DNA 分析相结合可能提供重要信息，但由于这两种测试是独立的，它们可能无法关联。最近，对 RNA 转录本中的编码区 SNP（cSNP）进行分析已被证明是应对这一挑战的一种有前途的方法。在这项研究中，我们使用 Ion S5™ 系统和 BFID-cSNP-6F 检测试剂盒对 12 对夫妇在亲密接触后和非亲密接触后的不同时间点收集的 158 个样本（拳击短裤、指甲拭子和阴茎拭子）进行了靶向 RNA 测序。该研究的目的是比较 MPS 和 CE 方法在检测 mRNA 标记物方面的性能，并通过 cSNP 基因型将体液与贡献者联系起来。研究结果表明，MPS 检测阴道黏膜的成功率低于 CE 方法。然而，cSNP 基因型提供的额外信息可以在大多数情况下成功地将体液与贡献者联系起来。
