自噬相关 microRNA：肿瘤 miR-125b 与甲状腺癌。
Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers.
机构信息
Ministry of Health of Russia, Siberian State Medical University, 634050 Tomsk, Russia.
Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, 634050 Tomsk, Russia.
出版信息
Genes (Basel). 2023 Mar 9;14(3):685. doi: 10.3390/genes14030685.
BACKGROUND
Autophagy is a stress response mechanism that causes cellular components to degrade. Its defects were associated with multiple pathologies, including cancers. Thyroid cancer is known to be the most prevalent form of malignant neoplasm among endocrine tumors. The aim of the study was to seek and comprehensively explore the role of autophagy related genes and proteins play in thyroid cancers through bioinformatics analysis with their detection in the tissue samples.
METHODS
Bioinformatics analysis was performed to investigate autophagy related proteins and genes involvement in thyroid cancer progression. The experimental verification was done in cancer samples of one hundred and three patients with thyroid pathology included in the study. The miR-125blevel was detected by PCR in real time.
RESULTS AND DISCUSSION
The bioinformatics analysis verified the miR-125b as a regulatory mechanism in autophagy. Its expression in patients with PTC was reduced by 6.75 times in cancer patients compared to the patients with benign tumors. The BRAFV600E mutations were associated with a decrease in hsa-miR-125b expression by 12.67 times compared to tumors with the wild-type gene.
CONCLUSIONS
Our findings revealed involvement of the autophagy related proteins in cancer progression. The significant mechanisms of regulation are non-coding RNA sequences implicated in a variety of oncogenic processes. We found that miR-125b is a potential maker in thyroid cancer invasion, BRAV600E mutational status and risk of recurrence.
背景
自噬是一种导致细胞成分降解的应激反应机制。其缺陷与多种病理学有关，包括癌症。甲状腺癌是内分泌肿瘤中最常见的恶性肿瘤。本研究的目的是通过生物信息学分析，寻找并全面探讨自噬相关基因和蛋白在甲状腺癌中的作用，并在组织样本中检测它们。
方法
通过生物信息学分析研究自噬相关蛋白和基因在甲状腺癌进展中的作用。在研究中，对 103 例甲状腺病理患者的癌症样本进行了实验验证。通过实时 PCR 检测 miR-125b 水平。
结果与讨论
生物信息学分析验证了 miR-125b 作为自噬的调控机制。与良性肿瘤患者相比，PTC 患者的 miR-125b 表达降低了 6.75 倍。与野生型基因相比，BRAFV600E 突变使 hsa-miR-125b 表达降低了 12.67 倍。
结论
我们的研究结果揭示了自噬相关蛋白在癌症进展中的作用。调控的重要机制是非编码 RNA 序列，参与多种致癌过程。我们发现 miR-125b 是甲状腺癌侵袭、BRAV600E 突变状态和复发风险的潜在标志物。
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