Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw, Poland.
Laboratory of Molecular Biology, Vet-Lab Brudzew, ul. Turkowska 58c, 62-720 Brudzew, Poland.
Int J Mol Sci. 2023 Mar 7;24(6):5135. doi: 10.3390/ijms24065135.
is a common foodborne infection. Many serovars belonging to subsp. are present in the gut of various animal species. They can cause infection in human infants via breast milk or cross-contamination with powdered milk. In the present study, BO was isolated from human milk in accordance with ISO 6579-1:2017 standards and sequenced using whole-genome sequencing (WGS), followed by serosequencing and genotyping. The results also allowed its pathogenicity to be predicted. The WGS results were compared with the bacterial phenotype. The isolated strain was found to be subsp. serovar Typhimurium 4:i:1,2_69M ( Typhimurium 69M); it showed a very close similarity subsp. serovar Typhimurium LT2. Bioinformatics sequence analysis detected eleven SPIs (SPI-1, SPI-2, SPI-3, SPI-4, SPI-5, SPI-9, SPI-12, SPI-13, SPI-14, C63PI, CS54_island). Significant changes in gene sequences were noted, causing frameshift mutations in , , , , (insertion) and , , , (deletion). The sequences of several proteins were significantly different from those coded in the reference genome; their three-dimensional structure was predicted and compared with reference proteins. Our findings indicate the presence of a number of antimicrobial resistance genes that do not directly imply an antibiotic resistance phenotype.
是一种常见的食源性感染。许多属于 subsp. 的血清型存在于各种动物物种的肠道中。它们可以通过母乳或与奶粉的交叉污染感染人类婴儿。在本研究中，根据 ISO 6579-1:2017 标准从人乳中分离出 BO，并进行全基因组测序 (WGS)，随后进行血清测序和基因分型。结果还允许预测其致病性。将 WGS 结果与细菌表型进行比较。分离株被鉴定为 subsp. 血清型 Typhimurium 4:i:1,2_69M (Typhimurium 69M)；它与 subsp. 血清型 Typhimurium LT2 非常相似。生物信息学序列分析检测到十一个 SPI (SPI-1、SPI-2、SPI-3、SPI-4、SPI-5、SPI-9、SPI-12、SPI-13、SPI-14、C63PI、CS54_island)。基因序列发生了显著变化，导致 、 、 、 （插入）和 、 、 （缺失）发生移码突变。一些蛋白质的序列与参考基因组中编码的序列明显不同；预测并比较了它们的三维结构。我们的研究结果表明存在许多不直接暗示抗生素耐药表型的抗微生物药物耐药基因。
