Little R R, McKenzie E M, Wiedmeyer H M, England J D, Goldstein D E
Clin Chem. 1986 May;32(5):869-71.
We present data on a filter-paper collection and assay method for measurement of glycated hemoglobin (gHb). Onto filter paper dipped into a solution of glucose oxidase (EC 1.1.3.4) and allowed to dry, approximately 20 microL of capillary blood was spotted. For analysis, we eluted the dried blood spot from the paper by soaking in water for 1 h, then measured the gHb in the eluate by affinity chromatography. Because the gHb significantly increased from day 1 to day 14 of storage, it was necessary to standardize the day of elution from the paper. We found a high correlation between gHb measured from samples stored for 14 days on treated paper and gHb measured by affinity chromatography from frozen whole blood or hemolysates (r = 0.96). This method is convenient, requiring small amounts of blood and little sample handling and assay time, and may be particularly useful in certain situations such as large-scale screening for diabetes.
我们展示了一种用于测量糖化血红蛋白（gHb）的滤纸采集和检测方法的数据。将滤纸浸入葡萄糖氧化酶（EC 1.1.3.4）溶液中，待其干燥后，滴加约20微升毛细血管血。分析时，我们将滤纸上的干血斑用水浸泡1小时进行洗脱，然后通过亲和色谱法测量洗脱液中的gHb。由于储存第1天到第14天gHb显著增加，因此有必要规范从滤纸上洗脱的日期。我们发现，在处理过的滤纸上储存14天的样本所测得的gHb与通过亲和色谱法从冷冻全血或溶血产物中测得的gHb之间存在高度相关性（r = 0.96）。该方法简便，所需血量少，样本处理和检测时间短，在某些情况下，如糖尿病大规模筛查中可能特别有用。
