Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China.
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266061, China.
Int J Mol Sci. 2023 Mar 17;24(6):5757. doi: 10.3390/ijms24065757.
Antarctic organisms are consistently suffering from multiple environmental pressures, especially the strong UV radiation caused by the loss of the ozone layer. The mosses and lichens dominate the vegetation of the Antarctic continent, which grow and propagate in these harsh environments. However, the molecular mechanisms and related regulatory networks of these Antarctic plants against UV-B radiation are largely unknown. Here, we used an integrated multi-omics approach to study the regulatory mechanism of long non-coding RNAs (lncRNAs) of an Antarctic moss () in response to UV-B radiation. We identified a total of 5729 lncRNA sequences by transcriptome sequencing, including 1459 differentially expressed lncRNAs (DELs). Through functional annotation, we found that the target genes of DELs were significantly enriched in plant-pathogen interaction and the flavonoid synthesis pathway. In addition, a total of 451 metabolites were detected by metabonomic analysis, and 97 differentially change metabolites (DCMs) were found. Flavonoids account for 20% of the total significantly up-regulated metabolites. In addition, the comprehensive transcriptome and metabolome analyses revealed the co-expression pattern of DELs and DCMs of flavonoids. Our results provide insights into the regulatory network of lncRNA under UV-B radiation and the adaptation of Antarctic moss to the polar environments.
南极生物不断受到多种环境压力的影响，尤其是臭氧层损耗导致的强烈紫外线辐射。苔藓和地衣是南极大陆植被的主要组成部分，它们在这些恶劣的环境中生长和繁殖。然而，这些南极植物对 UV-B 辐射的分子机制和相关调控网络在很大程度上是未知的。在这里，我们使用整合的多组学方法研究了南极苔藓 () 长非编码 RNA（lncRNA）对 UV-B 辐射响应的调控机制。我们通过转录组测序共鉴定了 5729 条 lncRNA 序列，包括 1459 条差异表达的 lncRNA（DEL）。通过功能注释，我们发现 DEL 的靶基因显著富集在植物-病原体相互作用和类黄酮合成途径中。此外，代谢组学分析共检测到 451 种代谢物，发现 97 种差异变化的代谢物（DCM）。类黄酮占总显著上调代谢物的 20%。此外，综合转录组和代谢组分析揭示了 DEL 和类黄酮 DCM 的共表达模式。我们的研究结果为 UV-B 辐射下 lncRNA 的调控网络以及南极苔藓对极地环境的适应提供了新的见解。
