Wessels R A, Rogers B B, Ou C N, Alcorn R, Buffone G J
Clin Chem. 1986 May;32(5):903-6.
"High-performance" liquid chromatography (HPLC), applied to hemoglobin analysis, is decidely more sensitive and gives better resolution than do routine electrophoretic methods. Here we present a case with a rare double heterozygote hemoglobin S/LeporeBoston, originally diagnosed as homozygous hemoglobin S by routine electrophoretic methods. Using a gradient elution weak cation-exchange HPLC technique, we could separate hemoglobin S and hemoglobin LeporeBoston and make the correct diagnosis. This case demonstrates how HPLC can be a useful adjunct to routine electrophoresis.
“高效”液相色谱法（HPLC）应用于血红蛋白分析时，显然比常规电泳方法更灵敏，分辨率更高。在此，我们报告一例罕见的血红蛋白S/波士顿Lepore双重杂合子病例，该病例最初通过常规电泳方法被诊断为血红蛋白S纯合子。使用梯度洗脱弱阳离子交换HPLC技术，我们能够分离出血红蛋白S和血红蛋白波士顿Lepore，并做出正确诊断。该病例证明了HPLC如何能够成为常规电泳的有用辅助手段。
