Ho Myoung Jin, Park Hyun Jin, Kang Myung Joo
College of Pharmacy, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan 330-714, Chungnam, Republic of Korea.
Materials (Basel). 2023 Mar 13;16(6):2294. doi: 10.3390/ma16062294.
In this study, a neutral oil-incorporated liposomal system (lipo-oil-some, LOS) was designed to improve the skin absorption of ascorbic acid (Vit C), and the effects of an edge activator and neutral oil on the skin absorption of Vit C were evaluated. As components of the LOS system, sodium deoxycholate, polysorbate 80, and cholesterol were screened as edge activators, and camellia oil, tricaprylin, and grapeseed oil were employed as neutral oils. The LOS systems prepared by the ethanol injection method were spherical in shape, 130-350 nm in size, and had 4-27% Vit C loading efficiency (%). In a skin absorption study using a Franz diffusion cell mounted with porcine skin, the LOS system prepared with sodium deoxycholate (10 w/w% of phospholipid) exhibited 1.2-and 2.9-fold higher absorption than those prepared with polysorbate 80 and cholesterol, respectively. Moreover, the type of neutral oil had a marked effect on the absorption of Vit C; the liposomal system containing camellia oil provided 1.3 to 1.8 times higher flux (45.4 μg/cm∙h) than vesicles with tricaprylin or grapeseed oil, respectively. The optimized lipid nanocarrier is expected to be a promising tool for promoting the skin absorption of Vit C and improving its dermatological functions.
在本研究中，设计了一种含中性油的脂质体系统（脂质体-油，LOS）以提高抗坏血酸（维生素C）的皮肤吸收，并评估边缘活化剂和中性油对维生素C皮肤吸收的影响。作为LOS系统的成分，筛选了脱氧胆酸钠、聚山梨酯80和胆固醇作为边缘活化剂，并使用山茶油、三辛酸甘油酯和葡萄籽油作为中性油。通过乙醇注入法制备的LOS系统呈球形，大小为130-350nm，维生素C负载效率为4-27%（%）。在使用装有猪皮的Franz扩散池进行的皮肤吸收研究中，用脱氧胆酸钠（磷脂的10w/w%）制备的LOS系统的吸收分别比用聚山梨酯80和胆固醇制备的系统高1.2倍和2.9倍。此外，中性油的类型对维生素C的吸收有显著影响；含有山茶油的脂质体系统的通量（45.4μg/cm∙h）分别比含有三辛酸甘油酯或葡萄籽油的囊泡高1.3至1.8倍。优化后的脂质纳米载体有望成为促进维生素C皮肤吸收并改善其皮肤学功能的有前途的工具。
