Barash V, Gimmon Z, Shafrir E
Diabetes Res. 1986 Feb;3(2):97-101.
The effect of maternal hyperglycaemia on glycogen and triglyceride accumulation in the feto-placental unit of non-diabetic rats was studied. Hyperglycaemia was induced by continuous infusion of a 400 g/l glucose solution at the rate of 2-4 g/hr/kg, from day 18.5-20.5 of gestation. Hyperglycaemic mothers were hyperinsulinaemic; their fetuses were hyperglycaemic but their insulin levels were comparable with those of control pregnant rats (infused with a 50 g/l glucose solution at the same rate). Fetal pancreas insulin content in the hyperglycaemic fetuses was pronouncedly reduced. The hyperglycaemia produced an approximately 2-fold increase in placental glycogen content in association with increased activities of placental glycogen synthase and phosphorylase. Maternal serum triglycerides fell concomitant with the hyperglycaemia. Placental triglyceride content of hyperglycaemic rats did not change significantly, whereas up to a 2-fold increase in maternal and fetal liver triglyceride concentration was observed. There was no change in fetal and placental weight. Since we have shown previously an increase in both placental glycogen and triglycerides in diabetic rats with hyperglycaemia, concomitant with elevation of plasma triglycerides and free fatty acids, the present experiments demonstrate that these 2 factors causing placental glycogen and triglyceride accumulation can be dissociated. On the other hand, maternal and fetal liver triglycerides accumulate in the hyperglycaemic rats probably as a result of local de vovo lipogenesis.
研究了母体高血糖对非糖尿病大鼠胎儿-胎盘单位中糖原和甘油三酯积累的影响。从妊娠第18.5天至20.5天，以2 - 4克/小时/千克的速率持续输注400克/升葡萄糖溶液诱导高血糖。高血糖的母体胰岛素水平升高；其胎儿血糖升高，但其胰岛素水平与对照妊娠大鼠（以相同速率输注50克/升葡萄糖溶液）相当。高血糖胎儿的胰腺胰岛素含量明显降低。高血糖使胎盘糖原含量增加约2倍，同时胎盘糖原合酶和磷酸化酶的活性增强。母体血清甘油三酯随着高血糖而下降。高血糖大鼠的胎盘甘油三酯含量没有显著变化，而母体和胎儿肝脏甘油三酯浓度则观察到高达2倍的增加。胎儿和胎盘重量没有变化。由于我们之前已经表明，糖尿病大鼠高血糖时胎盘糖原和甘油三酯均增加，同时血浆甘油三酯和游离脂肪酸升高，本实验表明导致胎盘糖原和甘油三酯积累的这两个因素可以分开。另一方面，高血糖大鼠母体和胎儿肝脏甘油三酯的积累可能是由于局部从头脂肪生成。
