Shijo Seito, Tanaka Daiki, Sekiguchi Tetsushi, Ishihara Jun-Ichi, Takahashi Hiroki, Kobayashi Masashi, Shoji Shuichi
Major in Nanoscience and Nanoengineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 145-0065, Japan.
Research Organization for Nano & Life Innovation, Waseda University, 513 Tsurumakicho, Shinjuku, Tokyo 162-0041, Japan.
Micromachines (Basel). 2023 Mar 22;14(3):706. doi: 10.3390/mi14030706.
We developed a microfluidic device that enables selective droplet extraction from multiple droplet-trapping pockets based on dielectrophoresis. The device consists of a main microchannel, five droplet-trapping pockets with side channels, and drive electrode pairs appropriately located around the trapping pockets. Agarose droplets capable of encapsulating biological samples were successfully trapped in the trapping pockets due to the difference in flow resistance between the main and side channels. Target droplets were selectively extracted from the pockets by the dielectrophoretic force generated between the electrodes under an applied voltage of 500 V. During their extraction from the trapping pockets, the droplets and their contents were exposed to an electric field for 400-800 ms. To evaluate whether the applied voltage could potentially damage the biological samples, the growth rates of cells in the droplets, with and without a voltage applied, were compared. No significant difference in the growth rate was observed. The developed device enables the screening of encapsulated single cells and the selective extraction of target droplets.
我们开发了一种微流控装置，该装置能够基于介电泳从多个液滴捕获腔中选择性提取液滴。该装置由一个主微通道、五个带有侧通道的液滴捕获腔以及围绕捕获腔适当定位的驱动电极对组成。由于主通道和侧通道之间的流动阻力差异，能够封装生物样品的琼脂糖液滴成功捕获在捕获腔中。在500 V的施加电压下，通过电极之间产生的介电泳力从腔中选择性提取目标液滴。在从捕获腔中提取液滴的过程中，液滴及其内含物暴露于电场中400 - 800毫秒。为了评估施加的电压是否可能损害生物样品，比较了施加电压和未施加电压的液滴中细胞的生长速率。未观察到生长速率有显著差异。所开发的装置能够筛选封装的单细胞并选择性提取目标液滴。
