da Silva Ana Alves, Galego Lisete, Arraiano Cecília Maria
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Microorganisms. 2023 Mar 1;11(3):632. doi: 10.3390/microorganisms11030632.
The BolA-like protein family is widespread among prokaryotes and eukaryotes. BolA was originally described in as a gene induced in the stationary phase and in stress conditions. The BolA overexpression makes cells spherical. It was characterized as a transcription factor modulating cellular processes such as cell permeability, biofilm production, motility, and flagella assembly. BolA is important in the switch between motile and sedentary lifestyles having connections with the signaling molecule c-di-GMP. BolA was considered a virulence factor in pathogens such as Typhimurium and and it promotes bacterial survival when facing stresses due to host defenses. In , the BolA homologue IbaG is associated with resistance to acidic stress, and in , IbaG is important for animal cell colonization. Recently, it was demonstrated that BolA is phosphorylated and this modification is important for the stability/turnover of BolA and its activity as a transcription factor. The results indicate that there is a physical interaction between BolA-like proteins and the CGFS-type Grx proteins during the biogenesis of Fe-S clusters, iron trafficking and storage. We also review recent progress regarding the cellular and molecular mechanisms by which BolA/Grx protein complexes are involved in the regulation of iron homeostasis in eukaryotes and prokaryotes.
BolA样蛋白家族广泛存在于原核生物和真核生物中。BolA最初被描述为在稳定期和应激条件下诱导表达的基因。BolA的过表达使细胞呈球形。它被表征为一种转录因子，可调节细胞通透性、生物膜形成、运动性和鞭毛组装等细胞过程。BolA在游动和定居生活方式的转换中很重要，与信号分子环二鸟苷酸（c-di-GMP）有关。BolA在鼠伤寒沙门氏菌等病原体中被认为是一种毒力因子，它在面对宿主防御引起的应激时促进细菌存活。在[具体文献]中，BolA同源物IbaG与耐酸性应激有关，在[具体文献]中，IbaG对动物细胞定殖很重要。最近，研究表明BolA被磷酸化，这种修饰对BolA的稳定性/周转及其作为转录因子的活性很重要。结果表明，在铁硫簇的生物合成、铁运输和储存过程中，BolA样蛋白与CGFS型谷氧还蛋白（Grx）之间存在物理相互作用。我们还综述了关于BolA/Grx蛋白复合物参与真核生物和原核生物铁稳态调节的细胞和分子机制的最新进展。
