Zhang Kundi, Lu Mengyao, Qiu Yuxin, Zhu Xiaoxuan, Wang Hongwei, Huang Yan, Dong Hongjie, Gu Lichuan
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, China.
Microorganisms. 2023 Mar 8;11(3):695. doi: 10.3390/microorganisms11030695.
is the main pathogen that causes bacterial vaginosis. In the healthy vaginal microecological environment of a woman, the lactobacilli produce lactate and hydrogen peroxide to inhibit the growth of pathogens such as . The lack of lactobacilli results in a high pH and low hydrogen peroxide in the vagina which facilitate growth, leading to the imbalance of the vaginal microecology. In this study, lactate and hydrogen peroxide were added to a culture medium to simulate the co-culture of the lactobacilli and , and then the genes related to the stress response of were identified using transcriptomics and proteomics. It was indicated that, among all the upregulated genes, most of them encoded transporters associated with the efflux of harmful substances, and the majority of the downregulated genes were related to the biofilm formation and epithelial cell adhesion. This study may help find new drug targets for for the development of novel therapies for bacterial vaginosis.
是引起细菌性阴道病的主要病原体。在女性健康的阴道微生态环境中，乳酸杆菌产生乳酸和过氧化氢以抑制诸如等病原体的生长。乳酸杆菌的缺乏导致阴道pH值升高和过氧化氢含量降低，这有利于生长，导致阴道微生态失衡。在本研究中，将乳酸和过氧化氢添加到培养基中以模拟乳酸杆菌和的共培养，然后使用转录组学和蛋白质组学鉴定与应激反应相关的基因。结果表明，在所有上调基因中，大多数编码与有害物质外排相关的转运蛋白，而下调基因大多与生物膜形成和上皮细胞粘附有关。本研究可能有助于找到针对的新药物靶点，以开发治疗细菌性阴道病的新疗法。
