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从发酵高粱(廷)浆中回收潜在发酵剂和益生菌。

Recovery of Potential Starter Cultures and Probiotics from Fermented Sorghum (Ting) Slurries.

作者信息

Rapoo Seth Molamu, Budeli Phumudzo, Thaoge Mathoto Lydia

机构信息

Department of Biotechnology and Food Technology, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa.

出版信息

Microorganisms. 2023 Mar 9;11(3):715. doi: 10.3390/microorganisms11030715.

Abstract

Fermented foods are thought to provide a source of probiotics that promote gut health. Consequently, isolation and characterization of fermented food strains and their applications in a controlled fermentation process or as probiotics present a new facet in this area of research. Therefore, the current study sought to identify dominant strains in sorghum-fermented foods (ting) and characterize their probiotic potential in vitro. Recovered isolates were identified as , , , subsp , , , and subsp based on the their 16S rRNA sequences. Increased biomass was noted in seven out of nine under a low pH of 3 and a high bile concentration of 2% in vitro. Bactericidal activities of isolated LABs presented varying degrees of resistance against selected pathogenic bacteria ranging between (1.57 to 41 mm), (10 to 41 mm), and (11.26 to 42 mm) for 14028, ATTC 6538 and , respectively. Ampicillin, erythromycin, mupirocin, tetracycline and chloramphenicol were able to inhibit growth of all selected LABs. Thus, isolates recovered from ting partially satisfy the potential candidacy for probiotics by virtue of being more tolerant to acid and bile, antibacterial activity and antibiotic resistance.

摘要

发酵食品被认为是益生菌的一个来源,有助于促进肠道健康。因此,对发酵食品菌株进行分离、鉴定,并将其应用于可控发酵过程或作为益生菌,成为了该研究领域的一个新方向。因此,本研究旨在鉴定高粱发酵食品(廷)中的优势菌株,并在体外表征其益生菌潜力。根据16S rRNA序列,回收的分离株被鉴定为、、、亚种、、、和亚种。在体外pH值为3且胆汁浓度为2%的条件下,9株中有7株生物量增加。分离出的乳酸菌对选定病原菌的杀菌活性表现出不同程度的抗性,14028、ATCC 6538和的抑菌圈直径分别在(1.57至41毫米)、(10至41毫米)和(11.26至42毫米)之间。氨苄青霉素、红霉素、莫匹罗星、四环素和氯霉素能够抑制所有选定乳酸菌的生长。因此,从廷中回收的分离株由于对酸和胆汁具有更高的耐受性、抗菌活性和抗生素抗性,部分满足了作为益生菌的潜在候选条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb23/10054160/2cb723a37a08/microorganisms-11-00715-g001.jpg

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