Chemical Biology Research Group, Institute of Organic Chemistry, RCNS, Magyar Tudósok Krt. 2., H-1117 Budapest, Hungary.
Molecular Cell Biology Research Group, Institute of Enzymology, RCNS, Magyar Tudósok Krt. 2., H-1117 Budapest, Hungary.
Molecules. 2023 Mar 9;28(6):2521. doi: 10.3390/molecules28062521.
Organic anion transporting polypeptides (OATPs) were found to readily deliver membrane impermeable, tetrazine bearing fluorescent probes into cells. This feature was explored in OATP3A1 conditioned bio-orthogonal labeling schemes of various intracellular proteins in live cells. Confocal microscopy and super-resolution microscopy (STED) studies have shown that highly specific and efficient staining of the selected intracellular proteins can be achieved with the otherwise non-permeable probes when OATP3A1 is present in the cell membrane of cells. Such a transport protein linked bio-orthogonal labeling scheme is believed to be useful in OATP3A1 activity-controlled protein expression studies in the future.
有机阴离子转运多肽 (OATPs) 被发现可将膜不通透的、带有四嗪的荧光探针轻易递送至细胞内。本研究在 OATP3A1 条件下，探索了各种活细胞内蛋白的生物正交标记方案。共聚焦显微镜和超分辨率显微镜 (STED) 研究表明，当 OATP3A1 存在于细胞的细胞膜上时，即使是原本不可渗透的探针，也可以对选定的细胞内蛋白进行高度特异性和高效的染色。这种与转运蛋白相关的生物正交标记方案有望在未来的 OATP3A1 活性控制蛋白表达研究中发挥作用。
