Boothby J T, Jasper D E, Thomas C B
Cornell Vet. 1986 Apr;76(2):188-97.
The effect of vaccination on mycoplasmal infection and the cellular inflammatory response was evaluated in 4 vaccinated and 4 control cows experimentally challenged in 2 of 4 quarters with live Mycoplasma bovis. In unchallenged quarters during the first three weeks after experimental challenge exposure, 6 of 8 quarters on control cows, and 7 of 8 quarters on vaccinated cows became infected with low numbers (10(2)-10(4) cfu/ml) of M bovis. During the same period all challenge-infused quarters on both control and vaccinated animals became infected with high numbers (10(9) cfu/ml) of M bovis. Thereafter, all quarters on vaccinated cows became culture-negative for M bovis, while 2 of 8 unchallenged quarters, and 4 of 8 challenged quarters on 3 of 4 control cows remained infected. A cellular inflammatory response as measured by the California Mastitis Test accompanied the experimental infection in proportion to the infection level except in challenged quarters on vaccinated cows after the first three weeks post challenge in which the cellular inflammatory response remained high despite the advent of negative M bovis culture results. This study indicates that the course of experimental M bovis mastitis can be affected by vaccination, and that vaccination results in an adverse cellular inflammatory response in challenged quarters.
在4头接种疫苗的奶牛和4头对照奶牛身上评估了疫苗接种对支原体感染和细胞炎症反应的影响，这些奶牛在4个乳腺中的2个被用活的牛支原体进行实验性攻毒。在实验性攻毒暴露后的前三周内，对照奶牛8个乳腺中有6个，接种疫苗奶牛8个乳腺中有7个，在未攻毒的乳腺中感染了少量（10² - 10⁴ cfu/ml）的牛支原体。在同一时期，对照动物和接种疫苗动物所有攻毒注入的乳腺都感染了大量（10⁹ cfu/ml）的牛支原体。此后，接种疫苗奶牛的所有乳腺牛支原体培养均为阴性，而对照奶牛中8个未攻毒乳腺中的2个，以及4头对照奶牛中8个攻毒乳腺中的4个仍有感染。除了攻毒后前三周后接种疫苗奶牛的攻毒乳腺外，加利福尼亚乳房炎试验所测量的细胞炎症反应与实验性感染成比例，在攻毒乳腺中，尽管牛支原体培养结果为阴性，但细胞炎症反应仍然很高。这项研究表明，实验性牛支原体乳腺炎的病程会受到疫苗接种的影响，并且疫苗接种会在攻毒乳腺中导致不良的细胞炎症反应。
