Jakšić Daniela, Jelić Dubravko, Kopjar Nevenka, Šegvić Klarić Maja
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia.
Selvita, 10000 Zagreb, Croatia.
Pathogens. 2023 Mar 14;12(3):459. doi: 10.3390/pathogens12030459.
The most common Aspergilli isolated from indoor air samples from occupied buildings and a grain mill were extracted and analyzed for their combined ( + , + ) cytotoxic, genotoxic and pro-inflammatory properties on human adenocarcinoma cells (A549) and monocytic leukemia cells induced in macrophages (THP-1 macrophages). Metabolite mixtures from the Aspergilli series increase the cytotoxic and genotoxic potency of extracts in A549 cells suggesting additive and/or synergistic effects, while antagonizing the cytotoxic potency of extracts in THP-1 macrophages and genotoxicity in A549 cells. All tested combinations significantly decreased IL-5 and IL-17, while IL-1β, TNF-α and IL-6 relative concentrations were increased. Exploring the toxicity of extracted Aspergilli deepens the understanding of intersections and interspecies differences in events of chronic exposure to their inhalable mycoparticles.
从有人居住的建筑物和谷物加工厂的室内空气样本中分离出最常见的曲霉菌，并对其在人腺癌细胞（A549）和巨噬细胞诱导的单核细胞白血病细胞（THP-1巨噬细胞）上的联合（+，+）细胞毒性、遗传毒性和促炎特性进行提取和分析。来自曲霉菌系列的代谢物混合物增加了提取物在A549细胞中的细胞毒性和遗传毒性效力，表明存在相加和/或协同作用，同时拮抗提取物在THP-1巨噬细胞中的细胞毒性效力以及在A549细胞中的遗传毒性。所有测试组合均显著降低IL-5和IL-17，而IL-1β、TNF-α和IL-6的相对浓度则升高。探索提取出的曲霉菌的毒性，加深了我们对长期暴露于其可吸入真菌颗粒事件中的交叉点和种间差异的理解。
