Kruuv J
Cryobiology. 1986 Apr;23(2):126-33. doi: 10.1016/0011-2240(86)90003-9.
When multicellular spheroids, which simulate small bits of tissue, are exposed to a freeze-thaw (FT) cycle, the survival of the individual cells in the spheroid is higher if the cells of the spheroid are trypsinized and plated as single cells immediately after thawing than if the spheroid is allowed to remain intact for 4 hr and then trypsinized for plating. The results imply either that cell-to-cell contact inhibits repair of potentially lethal damage (PLD) or that accumulation of additional lethal or sublethal damage during the post-thaw period for cells in contact is taking place. Pre- and post-FT trypsinization of single cells indicate that trypsin does not enhance repair of PLD caused by a FT cycle.
当模拟小块组织的多细胞球体暴露于冻融（FT）循环时，如果球体细胞在解冻后立即用胰蛋白酶处理并铺板为单细胞，那么球体中单个细胞的存活率要高于让球体保持完整4小时然后再用胰蛋白酶处理用于铺板的情况。这些结果表明，要么细胞间接触会抑制潜在致死损伤（PLD）的修复，要么在解冻后期间，相互接触的细胞会积累额外的致死或亚致死损伤。对单细胞进行冻融前后的胰蛋白酶处理表明，胰蛋白酶不会增强由冻融循环引起的PLD的修复。
