Avila-Victor Consuelo Margarita, Ordaz-Chaparro Víctor Manuel, Arjona-Suárez Enrique de Jesús, Iracheta-Donjuan Leobardo, Gómez-Merino Fernando Carlos, Robledo-Paz Alejandrina
Colegio de Postgraduados, Campus Montecillo, Carretera México-Texcoco Km. 36.5, Montecillo, Texcoco C.P. 56264, Estado de México, Mexico.
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Rosario Izapa, Carretera Tapachula-Cacahoatán Km. 18, Tuxtla Chico C.P. 30870, Chiapas, Mexico.
Plants (Basel). 2023 Mar 8;12(6):1237. doi: 10.3390/plants12061237.
is one of the two most consumed coffee species in the world. Micropropagation through somatic embryogenesis has allowed the large-scale propagation of different coffee varieties. However, the regeneration of plants using this technique depends on the genotype. This study aimed to develop a protocol for the regeneration of L. var. Colombia by somatic embryogenesis for its mass propagation. Foliar explants were cultured on Murashige and Skoog (MS) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), and phytagel for inducing somatic embryogenesis. In total, 90% of the explants formed embryogenic calli with a culture medium containing 2 mg L of 2,4-D, 0.2 mg L BAP, and 2.3 g L phytagel. The highest number of embryos per gram of callus (118.74) was obtained in a culture medium containing 0.5 mg L 2,4-D, 1.1 mg L BAP, and 5.0 g L phytagel. In total, 51% of the globular embryos reached the cotyledonary stage when they were cultured on the growth medium. This medium contained 0.25 mg L BAP, 0.25 mg L indoleacetic acid (IAA), and 5.0 g L of phytagel. The mixture of vermiculite:perlite (3:1) allowed 21% of embryos to become plants.
是世界上消费最多的两种咖啡品种之一。通过体细胞胚胎发生进行微繁殖已实现了不同咖啡品种的大规模繁殖。然而，使用该技术进行植物再生取决于基因型。本研究旨在开发一种通过体细胞胚胎发生实现哥伦比亚卡蒂姆咖啡大规模繁殖的再生方案。将叶片外植体接种在添加了不同浓度的2,4-二氯苯氧乙酸（2,4-D）、6-苄基腺嘌呤（BAP）和植物凝胶的Murashige和Skoog（MS）培养基上，以诱导体细胞胚胎发生。总共，90%的外植体在含有2 mg/L 2,4-D、0.2 mg/L BAP和2.3 g/L植物凝胶的培养基上形成了胚性愈伤组织。在含有0.5 mg/L 2,4-D、1.1 mg/L BAP和5.0 g/L植物凝胶的培养基中，每克愈伤组织获得的胚胎数量最多（118.74个）。当将球形胚在含有0.25 mg/L BAP、0.25 mg/L吲哚乙酸（IAA）和5.0 g/L植物凝胶的生长培养基上培养时，总共51%的球形胚达到了子叶期。蛭石:珍珠岩（3:1）的混合物使21%的胚胎发育成植株。
