Herniter Ira A, Kim Yurah, Wang Yifei, Havill Joshua S, Johnson-Cicalese Jennifer, Muehlbauer Gary J, Iorizzo Massimo, Vorsa Nicholi
Department of Plant Biology, Rutgers University, New Brunswick, NJ 08901, USA.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA.
Plants (Basel). 2023 Mar 16;12(6):1346. doi: 10.3390/plants12061346.
Blueberries ( sect. ) are a dietary source of phenolic acids, including chlorogenic acid (CGA) and related compounds such as acetylated caffeoylquinic acid (ACQA) and caffeoylarbutin (CA). These compounds are known to be potent antioxidants with potential health benefits. While the chemistry of these compounds has been extensively studied, the genetic analysis has lagged behind. Understanding the genetic basis for traits with potential health implications may be of great use in plant breeding. By characterizing genetic variation related to fruit chemistry, breeders can make more efficient use of plant diversity to develop new cultivars with higher concentrations of these potentially beneficial compounds. Using a large interspecific F1 population, developed from a cross between the temperate var. and the subtropical , with 1025 individuals genotyped using genotype-by-sequencing methods, of which 289 were phenotyped for phenolic acid content, with data collected across 2019 and 2020, we have identified loci associated with phenolic acid content. Loci for the compounds clustered on the proximal arm of Vc02, suggesting that a single gene or several closely associated genes are responsible for the biosynthesis of all four tested compounds. Within this region are multiple gene models similar to hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyltransferase (HCT) and UDP glucose:cinnamate glucosyl transferase (UGCT), genes known to be involved in the CGA biosynthesis pathway. Additional loci on Vc07 and Vc12 were associated with caffeoylarbutin content, suggesting a more complicated biosynthesis of that compound.
蓝莓（部分品种）是酚酸的膳食来源，包括绿原酸（CGA）以及相关化合物，如乙酰化咖啡酰奎尼酸（ACQA）和咖啡酰熊果苷（CA）。已知这些化合物是具有潜在健康益处的强效抗氧化剂。虽然对这些化合物的化学性质已进行了广泛研究，但遗传分析却滞后了。了解具有潜在健康影响的性状的遗传基础可能在植物育种中非常有用。通过表征与果实化学相关的遗传变异，育种者可以更有效地利用植物多样性来培育这些潜在有益化合物浓度更高的新品种。我们利用一个大型种间F1群体（由温带品种和亚热带品种杂交培育而成），通过测序分型方法对1025个个体进行了基因分型，其中289个个体测定了酚酸含量，并在2019年和2020年收集了数据，我们已经确定了与酚酸含量相关的基因座。这些化合物的基因座聚集在Vc02的近端臂上，这表明单个基因或几个紧密相关的基因负责所有四种测试化合物的生物合成。在该区域内有多个与羟基肉桂酰辅酶A莽草酸/奎尼酸羟基肉桂酰转移酶（HCT）和UDP葡萄糖：肉桂酸葡萄糖基转移酶（UGCT）相似的基因模型，这些基因已知参与CGA生物合成途径。Vc07和Vc12上的其他基因座与咖啡酰熊果苷含量相关，这表明该化合物的生物合成更为复杂。
