Mao Qifen, Xia Xufen, Luo Hongbin, Jin Li, Li Ying, Zhu Jinjun, Wang Yuan, Shangguan Zuifei, Xu Jiangyan
Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China.
The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310006, China.
Adv Biol (Weinh). 2023 Oct;7(10):e2300009. doi: 10.1002/adbi.202300009. Epub 2023 Mar 29.
This study aims to investigate the relevant mechanism by which hydrazinocurcumin (HC) interferes with A431 cell autophagy by inhibiting the STAT3 signaling pathway. Different concentrations of HC are used to treat A431 cells to study the effects of HC on A431 cell proliferation and apoptosis. Real-time fluorescent quantitative polymerase chain reaction (PCR) is used to further explore the relationship of HC with the JAK signaling pathway and autophagy. Double immunofluorescence staining is used to detect the fluorescence localization of LC3 and STAT3 after HC treatment. With increasing HC concentrations, A431 cell viability decreases in a dose-dependent manner, and the apoptosis rate increases significantly. Laser confocal colocalization reveals that the fluorescence of labeled LC3 protein is significantly increased, and the fluorescence of labeled STAT3 is significantly reduced in this study. HC may induce autophagy in A431 cells and affect cell proliferation by downregulating the JAK/STAT3 signaling pathway.
本研究旨在探讨肼基姜黄素（HC）通过抑制信号转导和转录激活因子3（STAT3）信号通路干扰A431细胞自噬的相关机制。采用不同浓度的HC处理A431细胞，以研究HC对A431细胞增殖和凋亡的影响。运用实时荧光定量聚合酶链反应（PCR）进一步探究HC与Janus激酶（JAK）信号通路及自噬的关系。采用双重免疫荧光染色检测HC处理后微管相关蛋白1轻链3（LC3）和STAT3的荧光定位。随着HC浓度的增加，A431细胞活力呈剂量依赖性降低，凋亡率显著升高。激光共聚焦定位显示，在本研究中，标记的LC3蛋白荧光显著增加，而标记的STAT3荧光显著降低。HC可能通过下调JAK/STAT3信号通路诱导A431细胞自噬并影响细胞增殖。
