Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Tokyo, Japan.
Department of Fisheries and Aquaculture, Faculty of Fisheries and Marine Sciences and Technology, University of Ruhuna, Matara, Sri Lanka.
J Fish Dis. 2023 Jul;46(7):779-790. doi: 10.1111/jfd.13786. Epub 2023 Mar 29.
Vibrio nigripulchritudo causes vibriosis in penaeid shrimps. Here, we used Illumina and Nanopore sequencing technologies to sequence the genomes of three of its strains (TUMSAT-V. nig1, TUMSAT-V. nig2, and TUMSAT-V. nig3) to explore opportunities for disease management. Putative virulence factors and mobile genetic elements were detected while evaluating the phylogenetic relationship of each isolated strain. The genomes consisted of two circular chromosomes (I and II) plus one or two plasmids. The size of chromosome I ranged from 4.02 to 4.07 Mb with an average GC content of 46%, while the number of predicted CDSs ranged from 3563 to 3644. The size of chromosome II ranged from 2.16 to 2.18 Mb, with an average GC content of 45.5%, and the number of predicted CDSs ranged from 1970 to 1987. Numerous virulence genes were identified related to adherence, antiphagocytosis, chemotaxis, motility, iron uptake, quorum sensing, secretion systems, and toxins in all three genomes. Higher numbers of prophages and genomic islands found in TUMSAT-V. nig1 suggest that the strain has experienced numerous horizontal gene transfer events. The presence of antimicrobial resistance genes suggests that the strains have multidrug resistance. Comparative genomic analysis showed that all three strains belonged to the same clade.
乌贼虫 Nigripulchritudo 引起对虾的弧菌病。在这里，我们使用 Illumina 和 Nanopore 测序技术对其三个菌株（TUMSAT-V. nig1、TUMSAT-V. nig2 和 TUMSAT-V. nig3）进行基因组测序，以探索疾病管理的机会。在评估每个分离菌株的系统发育关系时，检测了假定的毒力因子和移动遗传元件。基因组由两个圆形染色体（I 和 II）加一个或两个质粒组成。染色体 I 的大小范围为 4.02 到 4.07 Mb，平均 GC 含量为 46%，而预测的 CDS 数量范围为 3563 到 3644。染色体 II 的大小范围为 2.16 到 2.18 Mb，平均 GC 含量为 45.5%，预测的 CDS 数量范围为 1970 到 1987。在所有三个基因组中都鉴定出了与粘附、抗吞噬、趋化性、运动性、铁摄取、群体感应、分泌系统和毒素相关的大量毒力基因。在 TUMSAT-V. nig1 中发现了更多的前噬菌体和基因组岛，表明该菌株经历了多次水平基因转移事件。存在抗生素耐药基因表明菌株具有多药耐药性。比较基因组分析表明，所有三个菌株都属于同一进化枝。
