Di Xuerong, Fu Yutong, Xu Yingming, Zheng Shunan, Huang Qingqing, Sun Yuebing
Key Laboratory of Original Agro‒Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs (MARA), Agro‒Environmental Protection Institute, MARA/ Tianjin Key Laboratory of Agro‒Environment and Agro‒Product Safety, MARA, Tianjin, 300191, China.
Rural Energy & Environment Agency, MARA, Beijing, 100125, China.
Plant Physiol Biochem. 2023 Apr;197:107657. doi: 10.1016/j.plaphy.2023.107657. Epub 2023 Mar 23.
In this study, a soil culture and a hydroponic experiment were conducted to assess the toxicology effects of copper oxide nanoparticles (CuO NPs) on soil microbial community structure and the growth of bok choy. Results showed CuO NPs had an inhibitory effect on soil microbial abundance, diversity, and activity, as well as the bok choy seedling growth, whereas CuO NPs at low concentrations did not significantly affect the soil microbial biomass or plant growth. In soil, CuO NPs at high dose (80 mg kg) significantly reduced the indexes of Simpson diversity, Shannon-Wiener diversity and Pielou evenness by 3.7%, 4.9% and 4.5%, respectively. In addition, CuO NPs at 20 and 80 mg kg treatment significantly reduced soil enzymes (urease, alkaline phosphatase, dehydrogenase, and catalase) activities by 25.5%-58.9%. Further, CuO NPs at 20 mg L significantly inhibited the growth of plant root by 33.8%, and catalase (CAT) activity by 17.9% in bok choy seedlings. The present study can provide a basis for a comprehensive evaluation of the toxicity effect of CuO NPs on soil microorganisms and phytotoxicity to bok choy seedlings.
在本研究中，进行了土壤培养和水培实验，以评估氧化铜纳米颗粒（CuO NPs）对土壤微生物群落结构和小白菜生长的毒理学效应。结果表明，CuO NPs对土壤微生物丰度、多样性和活性以及小白菜幼苗生长具有抑制作用，而低浓度的CuO NPs对土壤微生物生物量或植物生长没有显著影响。在土壤中，高剂量（80 mg/kg）的CuO NPs使辛普森多样性指数、香农-威纳多样性指数和皮洛均匀度指数分别显著降低了3.7%、4.9%和4.5%。此外，20和80 mg/kg处理的CuO NPs使土壤酶（脲酶、碱性磷酸酶、脱氢酶和过氧化氢酶）活性显著降低了25.5%-58.9%。进一步研究发现，20 mg/L的CuO NPs显著抑制了植物根系生长33.8%，并使小白菜幼苗中的过氧化氢酶（CAT）活性降低了17.9%。本研究可为全面评估CuO NPs对土壤微生物的毒性效应及对小白菜幼苗的植物毒性提供依据。
