深入了解 FBH1 与 PCNA 的结合模式。
Molecular insight into the PCNA-binding mode of FBH1.
机构信息
Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, 28040 Madrid, Spain.
出版信息
Structure. 2023 May 4;31(5):511-517.e3. doi: 10.1016/j.str.2023.03.004. Epub 2023 Mar 28.
Abstract
F-box DNA helicase 1 (FBH1) is involved in the regulation of cell responses to replicative stress. FBH1 is recruited to stalled DNA replication fork by PCNA where it inhibits homologous recombination and catalyzes fork regression. Here, we report the structural basis for the molecular recognition of two distinctly different motifs of FBH1, FBH1 and FBH1, by PCNA. The crystal structure of PCNA in complex with FBH1 and analysis of NMR perturbations reveal overlapped FBH1 and FBH1 binding sites of PCNA and the dominant contribution of FBH1 in this interaction.
摘要
F-box DNA 解旋酶 1（FBH1）参与调节细胞对复制应激的反应。FBH1 通过 PCNA 被招募到停滞的 DNA 复制叉处，在那里它抑制同源重组并催化叉回归。在这里，我们报告了 PCNA 与 FBH1 和 FBH1 两种截然不同的基序进行分子识别的结构基础。PCNA 与 FBH1 复合物的晶体结构和 NMR 扰动分析揭示了 PCNA 上重叠的 FBH1 和 FBH1 结合位点，以及 FBH1 在这种相互作用中的主要贡献。
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