Wagemaker G, Visser T P
Exp Hematol. 1986 May;14(4):303-6.
Heterozygous alpha-thalassemic (Hbath/+) female mice were investigated for the effect of persistent erythropoietic stress on the number of stem cells and progenitor cells along the the erythroid (E), granulocyte-macrophage (GM), and megakaryocyte (Meg) pathways. At the progenitor cell level, compensatory erythropoiesis was demonstrated in the spleen but not in the bone marrow. In the spleen, developmentally early progenitor cells (BFU-E) were expanded two- to threefold and late progenitor cells (CFU-E) five- to sixfold. A comparable expansion of progenitor cells was observed along the GM and Meg pathways. CFU-S numbers were increased in the spleen, but not in the bone marrow. The increases in GM and Meg progenitor cells appeared to result in an inappropriate hemopoiesis: peripheral thrombocyte and monocyte numbers were elevated. However, granulocyte numbers were not significantly increased. It is concluded that the persistently increased erythropoietic demand results in inappropriate production of other hemopoietic cells, most likely because pathway-specific regulatory mechanisms do not influence differentiation at the stem cell level.
对杂合子α地中海贫血（Hbath/+）雌性小鼠进行了研究，以探讨持续性红细胞生成应激对沿红系（E）、粒细胞-巨噬细胞系（GM）和巨核细胞系（Meg）途径的干细胞和祖细胞数量的影响。在祖细胞水平，脾脏中出现了代偿性红细胞生成，而骨髓中未出现。在脾脏中，发育早期祖细胞（BFU-E）扩增了2至3倍，晚期祖细胞（CFU-E）扩增了5至6倍。沿GM和Meg途径观察到祖细胞有类似的扩增。脾脏中CFU-S数量增加，但骨髓中未增加。GM和Meg祖细胞的增加似乎导致了不适当的造血：外周血小板和单核细胞数量升高。然而，粒细胞数量没有显著增加。得出的结论是，持续增加的红细胞生成需求导致其他造血细胞的不适当产生，最可能的原因是特定途径的调节机制在干细胞水平上不影响分化。
