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口虾蛄血淋巴(甲壳纲,口足目)中卵黄生成蛋白的免疫学特性

Immunological characterization of the vitellogenic proteins in Squilla mantis hemolymph (Crustacea, Stomatopoda).

作者信息

Marzari R, Ferrero E, Mosco A, Savoini A

出版信息

Exp Biol. 1986;45(2):75-80.

PMID:3699131
Abstract

The femal specific proteins (FSP) identified in the hemolymph of Squilla mantis females are here proven to be vitellogenins (VTG) by immunological and electrophoretical methods. VTG 1 which has a higher molecular weight (MW) and only one central form, both in hemolymph and eggs, can be detected immunoelectrophoretically. VTG 2 and 3 have similar lower MW and are immunologically closely related, their slightly cathodic precipitation are fusing with the slightly anodic form. A further cathodic shift of the double arc occurs in the corresponding egg proteins. The VTGs are forms of yolk proteins which are transported in the hemolymph from the site of synthesis to the ovary where they undergo structural variations without involving a change of MW or of their number.

摘要

通过免疫学和电泳方法已证实,在雌性口虾蛄血淋巴中鉴定出的雌性特异性蛋白(FSP)为卵黄蛋白原(VTG)。通过免疫电泳可检测到VTG 1,其分子量较高,且在血淋巴和卵中均只有一种中心形式。VTG 2和VTG 3具有相似的较低分子量,且在免疫学上密切相关,它们略微向阴极的沉淀与略微向阳极的形式融合。在相应的卵蛋白中,双弧进一步向阴极移动。VTG是卵黄蛋白的形式,它们在血淋巴中从合成部位运输到卵巢,在那里它们经历结构变化,但分子量和数量不变。

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