人类中性粒细胞NADPH氧化酶制剂中细胞色素b - 245的存在。
Presence of cytochrome b-245 in NADPH oxidase preparations from human neutrophils.
作者信息
Bellavite P, Cassatella M A, Papini E, Megyeri P, Rossi F
出版信息
FEBS Lett. 1986 Apr 21;199(2):159-63. doi: 10.1016/0014-5793(86)80471-9.
PMID:3699149
Abstract
The composition of NADPH oxidase purified by Red Sepharose chromatography of extracts from human neutrophil membranes was investigated. In contrast to that was recently reported by others, the enzyme isolated according to this procedure contained a high concentration of cytochrome b-245 and little FAD. The results reinforce the belief that cytochrome b-245 is a major component of the NADPH oxidase and plays a fundamental role in the formation of O2-by neutrophils.
摘要
对通过红琼脂糖层析法从人中性粒细胞膜提取物中纯化的NADPH氧化酶的组成进行了研究。与其他人最近报道的情况相反，按照此程序分离的酶含有高浓度的细胞色素b-245且FAD含量很少。这些结果强化了一种观点，即细胞色素b-245是NADPH氧化酶的主要成分，并且在中性粒细胞形成超氧阴离子的过程中起基本作用。
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