Politecnico di Milano, 20133 Milano, Italy.
CNRS, Institut FOTON, Université de Rennes, UMR 6082, F-22305 Lannion, France.
Sensors (Basel). 2023 Mar 7;23(6):2911. doi: 10.3390/s23062911.
Diffuse reflectance spectroscopy (DRS) has proven to be a powerful, reliable, and non-invasive optical method for characterizing a specimen. Nevertheless, these methods are based on a rudimentary interpretation of the spectral response and can be irrelevant to understanding 3D structures. In this work, we proposed adding optical modalities into a customized handheld probe head in order to increase the number of parameters in DRS acquired from the light/matter interaction. It consists of (1) placing the sample in a reflectance manual rotation stage to collect spectral backscattered angularly resolved light and (2) illuminating it with two sequential linear polarization orientations. We demonstrate that this innovative approach leads to a compact instrument, capable of performing fast polarization-resolved spectroscopic analysis. Due to the significant amount of data available with this technique in a short time, we observe sensitive quantitative discrimination between two types of biological tissue provided by a raw rabbit leg. We believe that this technique can pave the way for rapid meat quality check or biomedical diagnosis of pathological tissues in situ at an early stage.
漫反射光谱（DRS）已被证明是一种强大、可靠且非侵入性的光学方法，可用于对样本进行特征描述。然而，这些方法基于对光谱响应的基本解释，可能与理解 3D 结构无关。在这项工作中，我们提出在定制的手持式探头中增加光学模式，以增加从光/物质相互作用中获取的 DRS 的参数数量。它包括（1）将样品放置在反射手动旋转台上，以收集角度分辨的光谱背散射光，（2）用两个连续的线性偏振方向对其进行照明。我们证明了这种创新方法可实现一种紧凑的仪器，能够进行快速的偏振分辨光谱分析。由于该技术在短时间内提供了大量数据，我们观察到两种类型的生物组织之间的敏感定量区分，这两种组织由一只未经处理的兔子腿提供。我们相信，这项技术可以为快速进行肉类质量检查或在早期对原位病理性组织进行生物医学诊断铺平道路。
