Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.
Department of Pathology, Louisiana State University School of Medicine, New Orleans, LA 70112, USA.
Viruses. 2023 Mar 15;15(3):755. doi: 10.3390/v15030755.
The human neurotropic JCPyV is the widespread opportunistic causative pathogen of the fatal demyelinating disease progressive multifocal leukoencephalopathy; however, it has also been implicated in the oncogenesis of several types of cancers. It causes brain tumors when intracerebrally inoculated into rodents, and genomic sequences of different strains and expression of the viral protein large T-Antigen have been detected in a wide variety of glial brain tumors and CNS lymphomas. Here, we present a case of an AIDS-related multifocal primary CNS lymphoma in which JCPyV genomic sequences of the three regions of JCPyV and expression of T-Antigen were detected by PCR and immunohistochemistry, respectively. No capsid proteins were detected, ruling out active JCPyV replication. Sequencing of the control region revealed that Mad-4 was the strain of JCPyV present in tumor cells. In addition, expression of viral proteins LMP and EBNA-1 from another ubiquitous oncogenic virus, Epstein-Barr, was also detected in the same lymphocytic neoplastic cells, co-localizing with JCPyV T-Antigen, suggesting a potential collaboration between these two viruses in the process of malignant transformation of B-lymphocytes, which are the site of latency and reactivation for both viruses.
人类神经嗜性 JCPyV 是广泛存在的机会性致病病原体，可引起致命的脱髓鞘疾病进行性多灶性脑白质病；然而，它也与多种类型癌症的发生有关。当将其脑内接种到啮齿动物中时，它会引起脑瘤，并且已经在各种神经胶质瘤和中枢神经系统淋巴瘤中检测到不同株的基因组序列和病毒蛋白大 T 抗原的表达。在这里，我们提出了一例 AIDS 相关的多灶性原发性中枢神经系统淋巴瘤病例，其中通过 PCR 和免疫组织化学分别检测到 JCPyV 的三个区域的 JCPyV 基因组序列和 T 抗原的表达。未检测到衣壳蛋白，排除了 JCPyV 的复制活性。对控制区的测序表明，Mad-4 是存在于肿瘤细胞中的 JCPyV 株。此外，另一种普遍存在的致癌病毒 Epstein-Barr 的病毒蛋白 LMP 和 EBNA-1 的表达也在同一淋巴细胞性肿瘤细胞中被检测到，与 JCPyV T 抗原共定位，表明这两种病毒在 B 淋巴细胞恶性转化过程中可能存在协同作用，B 淋巴细胞是这两种病毒潜伏和再激活的部位。
