Ahmed Ahmed Ezzat, Sindi Ramya Ahmad, Yousef Nasra Ahmed, Hussein Hassan A, Badr Magdy R, Syaad Khalid M Al, Al-Saeed Fatimah A, Hassaneen Ahmed Saad A, Abdelrahman Mohamed, Ali Montaser Elsayed
Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
Department of Theriogenology, Obstetrics, and Artificial Insemination, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Front Vet Sci. 2023 Mar 13;10:1138220. doi: 10.3389/fvets.2023.1138220. eCollection 2023.
The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities.
本研究调查了向卵母细胞成熟、受精及培养基中添加表皮生长因子（EGF）和/或β-巯基乙醇（βME）对水牛胚胎生产的影响。采集卵巢并在2小时内转运至实验室。从直径3至8毫米的卵泡中吸出卵丘卵母细胞复合体。首先，分别向成熟培养基（TCM-199）、受精培养基（IVF-TALP）或培养培养基（IVC：SOF）中添加0、10、20或50纳克/毫升的EGF，或0、25、50、100或200微摩尔的βME。我们的结果显示，向TCM-199、IVF-TALP或SOF培养基中添加20纳克/毫升的EGF可有效提高水牛胚胎的生长速率和发育，而50纳克/毫升的EGF仅在处理IVF-TALP和/或SOF培养基后能刺激胚胎生产，对IVM培养基则无此作用。然而，βME的效果不如EGF；当以50微摩尔的浓度添加到成熟和受精（IVF-TALP）培养基中时，它能刺激水牛胚胎的生长速率。其次，将20纳克/毫升的EGF和50微摩尔的βME作为有效浓度添加到成熟培养基中。与单独使用相比，EGF（20纳克/毫升）和βME（50微摩尔）联合处理对水牛胚胎没有显著的增强作用。从未来展望来看，需要进一步研究以考察联合使用EGF和βME对不同年龄类别和季节性地区的水牛卵母细胞成熟和受精的影响。
