Universidade Estadual de Londrina, Laboratório de Reprodução Animal, DCV-CCA-UEL, Londrina, Parana, Brazil; National Institute of Science and Technology for Dairy Production Chain (INCT-LEITE), Universidade Estadual de Londrina, Rodovia Celso Garcia Cid-Campus Universitário, PO Box 10011, Londrina, Parana, 86057-970, Brazil.
Universidade Estadual de Londrina, Laboratório de Reprodução Animal, DCV-CCA-UEL, Londrina, Parana, Brazil; National Institute of Science and Technology for Dairy Production Chain (INCT-LEITE), Universidade Estadual de Londrina, Rodovia Celso Garcia Cid-Campus Universitário, PO Box 10011, Londrina, Parana, 86057-970, Brazil.
Theriogenology. 2020 Jul 1;150:180-185. doi: 10.1016/j.theriogenology.2020.01.024. Epub 2020 Jan 14.
Among the reproductive biotechnologies, in vitro embryo production (IVEP) is an important tool for multiplying genetic material of superior merit. Recently, the number of embryos produced and transferred in vitro became significantly higher than that produced in vivo worldwide. In this context, the enhancement was attributable to ovum pick-up (OPU). With the advent of genomic technology, shortened breeding intervals, and increased selection accuracy, IVEP has attracted increasing attention for commercial use. The IVEP technique is well-established, but the embryo production rate has reached a plateau at 30-40%. Despite constant advances, the OPU/IVEP programs face some challenges that hinder the efficient application of the technique. Previous studies have shown that the quantity and quality of aspirated oocytes are essential factors for successful IVEP. This paper presents a brief overview of alternatives that can be employed to improve the process-seeking methods that assist in the recovery of better-quality oocytes and higher competence in OPU to improve embryo production. These strategies include using follicular wave synchronization prior to OPU, employing the influence of antral follicle populations, using the pre-OPU gonadotrophic stimulus and applying non-hormonal methods for selecting female donors.
在生殖生物技术中，体外胚胎生产（IVEP）是一种重要的工具，可以繁殖具有优异遗传物质的胚胎。最近，全球体外产生和转移的胚胎数量明显高于体内产生的胚胎。在这种情况下，这一增长归因于卵母细胞采集（OPU）。随着基因组技术的出现，缩短了繁殖间隔，并提高了选择的准确性，IVEP 越来越受到商业应用的关注。IVEP 技术已经成熟，但胚胎的生产效率已经达到 30-40%的高原。尽管不断取得进展，但 OPU/IVEP 计划仍面临一些挑战，这些挑战阻碍了该技术的有效应用。先前的研究表明，抽吸卵母细胞的数量和质量是 IVEP 成功的关键因素。本文简要概述了可用于改进该过程的替代方法，以寻求有助于回收质量更好的卵母细胞和提高 OPU 中胚胎生产能力的方法。这些策略包括在 OPU 前进行卵泡波同步化、利用窦卵泡群体的影响、在 OPU 前使用促性腺激素刺激和应用非激素方法选择雌性供体。
