Nolan Richard Brent, Bontrager Colleen, Bowser Abigail, Corley Alyssa, Fiedler Hana, Flathers Connor, Francis Lauren, Le Angel, Mahmoudjafari Seyyed, Nim Tinh, Muolo Connor E, Shores Brianna, Viermann Christopher, Waldren Adam, Zatezalo Carmen, Fan Jin-Yuan, Price Jeffrey L
Divsion of Biological and Biomedical Systems, School of Science and Engineering, University of Missouri - Kansas City, Kansas City, MO 64110, USA.
iScience. 2023 Mar 5;26(4):106343. doi: 10.1016/j.isci.2023.106343. eCollection 2023 Apr 21.
BRIDE OF DOUBLETIME (BDBT) interacts with the circadian kinase DOUBLETIME (DBT) and accumulates in eye foci during the dark of a light:dark cycle. BDBT foci are shown here to be broadly expressed in constant dark and low in constant light. Analysis of circadian photoreceptor and visual photoreceptor mutants showed that disappearance of eye BDBT foci requires both the CRYPTOCHROME and the RHODOPSIN-1 pathways. The and mutants, which affect rhodopsin quenching, eliminated BDBT foci under dark conditions. and mutants also caused increased nuclear PER protein. The changes in BDBT foci do not result from altered BDBT levels in the eye but from changes in its immunodetection. Knockdown of BDBT specifically in the eye produced constitutively nuclear PER and constitutively cytosolic DBT. The results show that BDBT is necessary for co-transport of DBT and PER into the nucleus and suggest that this process is regulated by a light-dependent mechanism.
双时新娘（BDBT）与昼夜节律激酶双时（DBT）相互作用，并在明暗周期的黑暗阶段在眼焦点中积累。在此显示，BDBT焦点在持续黑暗中广泛表达，而在持续光照下表达量较低。对昼夜节律光感受器和视觉光感受器突变体的分析表明，眼BDBT焦点的消失需要隐花色素和视紫红质-1途径。影响视紫红质淬灭的突变体和在黑暗条件下消除了BDBT焦点。突变体和还导致核PER蛋白增加。BDBT焦点的变化不是由眼中BDBT水平的改变引起的，而是由其免疫检测的变化引起的。特异性敲低眼中的BDBT会产生组成型核PER和组成型胞质DBT。结果表明，BDBT是DBT和PER共同转运到细胞核所必需的，并表明该过程受光依赖机制调节。
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