评估叶片提取物的抗氧化、神经保护、抗脲酶和抗酪氨酸酶能力。
assessment of antioxidant, neuroprotective, anti-urease and anti-tyrosinase capacities of leaves extracts.
机构信息
Biotechnology, Water, Environment and Health Laboratory, Faculty of Natural and Life Sciences, University Abbes Laghrour, Khenchela 40000, Algeria.
2 Laboratory of Natural Substances, Biomolecules and Biotechnological Applications, Faculty of Natural and Life Sciences, University Larbi Ben M Hidi, Oum El-Bouaghi 04000, Algeria.
出版信息
J Tradit Chin Med. 2023 Apr;43(2):252-264. doi: 10.19852/j.cnki.jtcm.20230105.003.
OBJECTIVE
To characterize the chemical profile of methanolic crude extract and its fractions (Ethyl acetate, n-butanol and aqueous) using liquid chromatography-mass spectrometry (LC-MS) analysis, to evaluate their biological and pharmacological properties: antioxidant (1, 1-diphenyl-2-pycrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic) (ABTS), galvinoxyle free radical scavenging, reducing power, phenanthroline and β carotene-linoleic acid bleaching assays), enzymes inhibitory ability against several enzymes [acetyl-cholinesterase (AChE), buthyrylcholinesterase (BChE), urease and tyrosinase].
METHODS
Secondary metabolites were extracted from Tamarix africana air-dried powdered leaves by maceration, the crude extract was fractionated using different solvents with different polarities (Ethyl acetate, n-butanol and aqueous). The amount of polyphenols, flavonoids and tannins (hydrolysable and condensed) were determined using colorimetric assays. A variety of biochemical tests were carried out to assess antioxidant and oxygen radical scavenging properties using DPPH, ABTS, galvinoxyle free radical scavenging, reducing power, phenanthroline and β carotene-linoleic acid bleaching methods. Neuroprotective effect was examined against acetylcholinesterase and buthy-rylcholinesterase enzymes. The anti-urease and anti-tyrosinase activities were performed against urease and tyrosinase enzymes respectively. The extract's components were identified using LC-MS and compared to reference substances.
RESULTS
The results indicated that Tamarix africana extracts presented a powerful antioxidant activity in all assays and exhibited a potent inhibitory effect against AChE and BChE as well as urease and tyrosinase enzymes. LC-MS analysis identified amount of eight phenolic compounds were revealed in this analysis; Apigenin, Diosmin, Quercetin, Quercetine-3-glycoside, Apigenin 7-O glycoside, Rutin, Neohesperidin and Wogonin in methanolic extract and its different fractions of Tamarix africana from leaves.
CONCLUSIONS
Based on these findings, it is reasonable to assume that Tamarix africana could be considered as a potential candidate for pharmaceutical, cosmetics, and food industries to create innovative health-promoting drugs.
目的
使用液相色谱-质谱联用（LC-MS）分析对 Tamarix africana 甲醇粗提物及其各馏分（乙酸乙酯、正丁醇和水）进行化学成分分析，并评估其生物和药理特性：抗氧化（1,1-二苯基-2-苦基肼基（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、Galvinoxyl 自由基清除、还原能力、邻菲啰啉和β-胡萝卜素-亚油酸漂白试验）、对几种酶（乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、脲酶和酪氨酸酶）的抑制能力。
方法
用浸渍法从 Tamarix africana 风干的粉状叶片中提取次生代谢物，用不同极性的溶剂（乙酸乙酯、正丁醇和水）对粗提物进行分级。使用比色法测定多酚、类黄酮和单宁（水解和缩合）的含量。使用 DPPH、ABTS、Galvinoxyl 自由基清除、还原能力、邻菲啰啉和β-胡萝卜素-亚油酸漂白法进行各种生化试验，以评估抗氧化和氧自由基清除特性。通过乙酰胆碱酯酶和丁酰胆碱酯酶酶试验检测神经保护作用。分别通过对脲酶和酪氨酸酶的抑制作用来检测抗脲酶和抗酪氨酸酶的活性。使用 LC-MS 对提取物的成分进行鉴定，并与对照物质进行比较。
结果
结果表明，Tamarix africana 提取物在所有试验中均表现出强大的抗氧化活性，并对 AChE 和 BChE 以及脲酶和酪氨酸酶表现出强大的抑制作用。LC-MS 分析共鉴定出八种酚类化合物；在 Tamarix africana 的甲醇提取物及其不同馏分中发现了芹菜素、地奥司明、槲皮素、槲皮素-3-糖苷、芹菜素 7-O 糖苷、芦丁、新橙皮苷和甘草素。
结论
基于这些发现，可以合理地假设 Tamarix africana 可被视为制药、化妆品和食品行业的潜在候选药物，以创造创新的促进健康的药物。
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