中胚层前体细胞的分离与鉴定技术进展。

State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts.

机构信息

Translational Cardiomyology Laboratory, Stem Cell and Developmental Biology Unit, Department of Development and Regeneration, KU Leuven, Leuven, Belgium.

Histology and Medical Embryology Unit, Department of Anatomy, Forensic Medicine and Orthopaedics, Sapienza University of Rome, Rome, Italy.

出版信息

Methods Mol Biol. 2023;2640:99-115. doi: 10.1007/978-1-0716-3036-5_8.

DOI:10.1007/978-1-0716-3036-5_8

PMID:36995590

Abstract

Adult skeletal muscle is a dynamic tissue able to regenerate quite efficiently, thanks to the presence of stem cell machinery. Besides the quiescent satellite cells that are activated upon injury or paracrine factors, other stem cells are described to be directly or indirectly involved in adult myogenesis. Mesoangioblasts (MABs) are vessel-associated stem cells originally isolated from embryonic dorsal aorta and, at later stages, from the adult muscle interstitium expressing pericyte markers. Adult MABs entered clinical trials for the treatment of Duchenne muscular dystrophy and the transcriptome of human fetal MABs has been described. In addition, single cell RNA-seq analyses provide novel information on adult murine MABs and more in general in interstitial muscle stem cells. This chapter provides state-of-the-art techniques to isolate and characterize murine MABs, fetal and adult human MABs.

摘要

成人骨骼肌是一种具有强大再生能力的动态组织，这要归功于其存在的干细胞机制。除了在损伤或旁分泌因子作用下被激活的静止卫星细胞外，还有其他干细胞被描述为直接或间接地参与成人肌肉发生。中胚层血管基质细胞（MABs）是最初从胚胎背主动脉中分离出来的血管相关干细胞，在后期，也可从成年肌肉间质中分离出来，这些细胞表达周细胞标志物。MABs 已进入治疗杜氏肌营养不良症的临床试验阶段，人类胎儿 MABs 的转录组也已被描述。此外，单细胞 RNA-seq 分析为成年鼠 MABs 以及更广泛的间质肌肉干细胞提供了新的信息。本章提供了分离和鉴定鼠 MABs、胎儿和成年人类 MABs 的最新技术。