Department of Muscle Development and Regeneration, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Methods Mol Biol. 2023;2640:207-215. doi: 10.1007/978-1-0716-3036-5_15.
Skeletal muscle is a highly plastic tissue that can alter its mass and strength in response to mechanical stimulation, such as overloading and unloading, which lead to muscle hypertrophy and atrophy, respectively. Mechanical loading in the muscle influences muscle stem cell dynamics, including activation, proliferation, and differentiation. Although experimental models of mechanical overloading and unloading have been widely used for the investigation of the molecular mechanisms regulating muscle plasticity and stem cell function, few studies have described the methods in detail. Here, we describe the appropriate procedures for tenotomy-induced mechanical overloading and tail-suspension-induced mechanical unloading, which are the most common and simple methods to induce muscle hypertrophy and atrophy in mouse models.
骨骼肌是一种高度可塑的组织，可以通过机械刺激（如超负荷和失负荷）改变其质量和力量，分别导致肌肉肥大和萎缩。肌肉中的机械负荷会影响肌肉干细胞的动力学，包括激活、增殖和分化。尽管机械超负荷和失负荷的实验模型已被广泛用于研究调节肌肉可塑性和干细胞功能的分子机制，但很少有研究详细描述这些方法。在这里，我们描述了跟腱切断术诱导的机械超负荷和尾部悬吊法诱导的机械失负荷这两种最常用、最简单的方法，这两种方法可在小鼠模型中诱导肌肉肥大和萎缩。
