利用CRISPR/Cas系统在芸苔属作物中进行高效靶向诱变

Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems.

作者信息

Lawrenson Tom, Youles Mark, Chhetry Monika, Clarke Martha, Harwood Wendy, Hundleby Penny

机构信息

John Innes Centre, Norwich, Norfolk, UK.

出版信息

Methods Mol Biol. 2023;2653:253-271. doi: 10.1007/978-1-0716-3131-7_16.

DOI:10.1007/978-1-0716-3131-7_16

PMID:36995631

Abstract

CRISPR/Cas has been established for targeted mutagenesis in many plant species since 2013, including Brassica napus and Brassica oleracea. Since that time, improvements have been made in terms of efficiency and choice of CRISPR systems. This protocol encompasses improved Cas9 efficiency and an alternative Cas12a system, allowing more challenging and diverse editing outcomes to be achieved.

摘要

自2013年以来，CRISPR/Cas已被用于多种植物物种的靶向诱变，包括甘蓝型油菜和甘蓝。从那时起，CRISPR系统在效率和选择方面都有了改进。本方案提高了Cas9的效率，并采用了另一种Cas12a系统，从而能够实现更具挑战性和多样化的编辑结果。