Jiang Qiantao, Yang Qiang, Harwood Wendy, Tang Huaping, Wei Yuming, Zheng Youliang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Methods Mol Biol. 2023;2653:287-296. doi: 10.1007/978-1-0716-3131-7_18.
Previous studies of gene function rely on the existing natural genetic variation or on induction of mutations by physical or chemical mutagenesis. The availability of alleles in nature, and random mutagenesis induced by physical or chemical means, limits the depth of research. The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system provides the means to rapidly modify genomes in a precise and predictable way, making it possible to modulate gene expression and modify the epigenome. Barley is the most appropriate model species for functional genomic analysis of common wheat. Therefore, the genome editing system of barley is very important for the study of wheat gene function. Here we detail a protocol for barley gene editing. The effectiveness of this method has been confirmed in our previous published studies.
以往对基因功能的研究依赖于现有的自然遗传变异或通过物理或化学诱变诱导突变。自然界中等位基因的可用性以及物理或化学手段诱导的随机诱变限制了研究的深度。CRISPR/Cas9（成簇规律间隔短回文重复序列/CRISPR相关蛋白9）系统提供了以精确且可预测的方式快速修饰基因组的手段，使得调节基因表达和修饰表观基因组成为可能。大麦是普通小麦功能基因组分析最合适的模式物种。因此，大麦的基因组编辑系统对于小麦基因功能的研究非常重要。在此，我们详细介绍一种大麦基因编辑方案。该方法的有效性已在我们之前发表的研究中得到证实。
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