Bryda Elizabeth C, Men Hongsheng, Stone Barbara J
University of Missouri, Rat Resource and Research Center, Columbia, MO, USA.
ParaTechs Corporation, Lexington, KY, USA.
Methods Mol Biol. 2023;2631:355-370. doi: 10.1007/978-1-0716-2990-1_16.
The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.
可进行基因操作的可靠的种系有能力的大鼠胚胎干细胞（ESC）系的可用性为生成新的大鼠模型提供了重要工具。在此，我们描述了培养大鼠胚胎干细胞、将胚胎干细胞显微注射到大鼠囊胚中，以及通过手术或非手术胚胎移植技术将胚胎转移到代孕母鼠体内以产生具有将基因修饰传递给其后代潜力的嵌合体动物的过程。
