Synytsya Alla, Janstová Daniela, Šmidová Miroslava, Synytsya Andriy, Petrtýl Jaromír
Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5;296:122664. doi: 10.1016/j.saa.2023.122664. Epub 2023 Mar 24.
Vibrational spectroscopic methods are widely used in the molecular diagnostics of carcinogenesis. Collagen, a component of connective tissue, plays a special role as a biochemical marker of pathological changes in tissues. The vibrational bands of collagens are very promising to distinguish between normal colon tissue, benign and malignant colon polyps. Differences in these bands indicate changes in the amount, structure, conformation and the ratio between the individual structural forms (subtypes) of this protein. The screening of specific collagen markers of colorectal carcinogenesis was carried out based on the FTIR and Raman (λ 785 nm) spectra of colon tissue samples and purified human collagens. It was found that individual types of human collagens showed significant differences in their vibrational spectra, and specific spectral markers were found for them. These collagen bands were assigned to specific vibrations in the polypeptide backbone, amino acid side chains and carbohydrate moieties. The corresponding spectral regions for colon tissues and colon polyps were investigated for the contribution of collagen vibrations. Mentioned spectral differences in collagen spectroscopic markers could be of interest for early ex vivo diagnosis of colorectal carcinoma if combine vibrational spectroscopy and colonoscopy.
振动光谱方法在癌症发生的分子诊断中被广泛应用。胶原蛋白是结缔组织的一种成分，作为组织病理变化的生化标志物发挥着特殊作用。胶原蛋白的振动带对于区分正常结肠组织、良性和恶性结肠息肉非常有前景。这些带的差异表明该蛋白质在数量、结构、构象以及各个结构形式（亚型）之间的比例上发生了变化。基于结肠组织样本和纯化的人胶原蛋白的傅里叶变换红外光谱（FTIR）和拉曼光谱（λ 785 nm），对结直肠癌发生的特定胶原蛋白标志物进行了筛选。研究发现，不同类型的人胶原蛋白在其振动光谱上表现出显著差异，并找到了针对它们的特定光谱标志物。这些胶原蛋白带被归因于多肽主链、氨基酸侧链和碳水化合物部分的特定振动。研究了结肠组织和结肠息肉的相应光谱区域中胶原蛋白振动的贡献。如果将振动光谱和结肠镜检查相结合，胶原蛋白光谱标志物中提到的光谱差异可能对结直肠癌的早期离体诊断具有重要意义。
