用于封装烟酰胺单核苷酸的卵清蛋白-岩藻多糖纳米颗粒的制备与评价：增强稳定性及抗衰老活性
Preparation and evaluation of ovalbumin-fucoidan nanoparticles for nicotinamide mononucleotide encapsulation with enhanced stability and anti-aging activity.
作者信息
Sun Shan, Zhang Xuedi, Li Jiaxuan, Li Yu, Zhou Chengfu, Xiang Siyuan, Tan Mingqian
机构信息
Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Gangjingzi District, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Gangjingzi District, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
出版信息
Food Chem. 2023 Aug 30;418:135982. doi: 10.1016/j.foodchem.2023.135982. Epub 2023 Mar 23.
Nicotinamide mononucleotide (NMN) has been recognized as a promising bio-active compound in relieving aging-related mitochondrial dysfunction. Self-assembled nanoparticles were prepared based on interaction between ovalbumin (OVA) and fucoidan to improve the stability and bio-accessibility of NMN. The OVA-fucoidan nanoparticles (OFNPs) displayed outstanding thermal stability and entrapment ability of NMN. The reactive oxygen species (ROS) analysis and senescence-associated β-galactosidase (SA-β-gal) staining characterization indicated that NMN encapsulated by OFNPs could effectively alleviate the cellular senescence of d-galactose-induced senescent cells. In vivo Caenorhabitis elegans experiment demonstrated that NMN-loaded OFNPs caused less accumulation of lipofuscin and protected NMN from thermal damage. Compared with free NMN, the NMN-loaded OFNPs prolonged lifespan from 28 to 31 days, increased 26% reproductive ability, and improved 12% body length of Caenorhabitis elegans. The results indicated that the use of nanocarriers could be a good strategy to improve anti-oxidative stress and anti-aging ability of NMN.
烟酰胺单核苷酸（NMN）已被公认为是一种在缓解衰老相关线粒体功能障碍方面颇具潜力的生物活性化合物。基于卵清蛋白（OVA）与岩藻依聚糖之间的相互作用制备了自组装纳米颗粒，以提高NMN的稳定性和生物可及性。OVA-岩藻依聚糖纳米颗粒（OFNPs）表现出出色的热稳定性和对NMN的包封能力。活性氧（ROS）分析和衰老相关β-半乳糖苷酶（SA-β-gal）染色表征表明，被OFNPs包封的NMN可有效减轻d-半乳糖诱导的衰老细胞的细胞衰老。体内秀丽隐杆线虫实验表明，负载NMN的OFNPs导致脂褐素积累减少，并保护NMN免受热损伤。与游离NMN相比，负载NMN的OFNPs使秀丽隐杆线虫的寿命从28天延长至31天，生殖能力提高26%，体长增加12%。结果表明，使用纳米载体可能是提高NMN抗氧化应激和抗衰老能力的良好策略。
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